Document Detail


Role of hyaluronic acid in maturation and further early embryo development of bovine oocytes.
MedLine Citation:
PMID:  22541325     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
Hyaluronic acid (HA), an important component of the extracellular matrix, plays a crucial role for cumulus cell expansion. Genes and proteins involved in HA synthesis and its receptor CD44 are expressed in cumulus oocyte complexes (COCs) in different animal species and increase during maturation. Hyaluronidase enzymes (Hyal) degrade HA into smaller biologically active HA fragments. To investigate the effects of the molecular size and concentration of HA on oocyte maturation and further embryo development, bovine oocytes were matured in vitro in the presence or absence of HA, Hyal-2 or 4-methylumbelliferone (4-MU); an HA synthesis inhibitor. The rates of oocyte nuclear maturation to metaphase II stage and development of embryos to blastocyst stage and blastocyst quality were recorded. Hyal-2 inhibited cumulus cell expansion without affecting oocyte maturation and further embryo development. Whereas, 4-MU at 1 mm reduced cumulus cell expansion, oocyte maturation rate and further embryo development; an effect which was partially abrogated by exogenous HA supplementation. These data suggest that HA production by cumulus cells during maturation is essential not only for cumulus cell expansion, but also for oocyte maturation and further embryo development. This effect is not affected by HA-degradation by Hyal-2.
Authors:
W F Marei; F Ghafari; A A Fouladi-Nashta
Related Documents :
20619345 - Downregulation of rhoa and changes in t cell cytoskeleton correlate with the abrogation...
20379865 - How t cells find their way around.
10854915 - Taste cell function. structural and biochemical implications.
19073935 - Optical methodology for detecting histologically unapparent nanoscale consequences of g...
6202645 - Plasminogen activators, activation inhibitors and alpha 2-macroglobulin produced by cul...
20732215 - Use of isolated lung cells in pulmonary toxicology.
Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2012-4-26
Journal Detail:
Title:  Theriogenology     Volume:  -     ISSN:  1879-3231     ISO Abbreviation:  -     Publication Date:  2012 Apr 
Date Detail:
Created Date:  2012-4-30     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0421510     Medline TA:  Theriogenology     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Copyright Information:
Copyright © 2012 Elsevier Inc. All rights reserved.
Affiliation:
Reproduction, genes and development group, Royal Veterinary College, Hawkshead Lane, North Mymms, Hatfield, Hertfordshire AL9 7TA, UK; Department of Theriogenology, Faculty of Veterinary Medicine, Cairo University, Giza 12211, Egypt.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Sperm cryopreservation affects postthaw motility, but not embryogenesis or larval growth in the Braz...
Next Document:  Computer assisted sperm analysis of motility patterns of postthawed epididymal spermatozoa of spring...