| Role of histone acetylation in reprogramming of somatic nuclei following nuclear transfer. | |
| | |
MedLine Citation:
|
PMID: 16481594 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
Before fertilization, chromatins of both mouse oocytes and spermatozoa contain very few acetylated histones. Soon after fertilization, chromatins of both gametes become highly acetylated. The same deacetylation-reacetylation changes occur with histones of somatic nuclei transferred into enucleated oocytes. The significance of these events in somatic chromatin reprogramming to the totipotent state is not known. To investigate their importance in reprogramming, we injected cumulus cell nuclei into enucleated mouse oocytes and estimated the histone deacetylation dynamics with immunocytochemistry. Other reconstructed oocytes were cultured before and/or after activation in the presence of the highly potent histone deacetylase inhibitor trychostatin A (TSA) for up to 9 h postactivation. The potential of TSA-treated and untreated oocytes to develop to the blastocyst stage and to full term was compared. Global deacetylation of histones in the cumulus nuclei occurred between 1 and 3 h after injection. TSA inhibition of histone deacetylation did not affect the blastocyst rate (37% with and 34% without TSA treatment), whereas extension of the TSA treatment beyond the activation point significantly increased the blastocyst rate (up to 81% versus 40% without TSA treatment) and quality (on average, 59 versus 45 cells in day 4 blastocysts with and without TSA treatment, respectively). TSA treatment also slightly increased full-term development (from 0.8% to 2.8%). Thus, deacetylation of somatic histones is not important for reprogramming, and hyperacetylation might actually improve reprogramming. |
| | |
Authors:
|
Andrei Rybouchkin; Yoko Kato; Yukio Tsunoda |
Related Documents
:
|
18477574 - Freezing within 2 h from oocyte retrieval increases the efficiency of human oocyte cryo... 15649474 - Aster self-organization at meiosis: a conserved mechanism in insect parthenogenesis? 9848314 - Repeated transmission of x-linked ocular albinism type 1 by a carrier oocyte donor. 20185514 - Embryo development and gestation using fresh and vitrified oocytes. 7026014 - Maternal nutrition in pregnancy. part ii: the implications of previous gastrointestinal... 20844814 - Serum beta human chorionic gonadotropin levels at 16 days following embryo transfer in ... |
Publication Detail:
|
Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2006-02-15 |
Journal Detail:
|
Title: Biology of reproduction Volume: 74 ISSN: 0006-3363 ISO Abbreviation: Biol. Reprod. Publication Date: 2006 Jun |
Date Detail:
|
Created Date: 2006-05-22 Completed Date: 2006-09-13 Revised Date: 2009-11-19 |
Medline Journal Info:
|
Nlm Unique ID: 0207224 Medline TA: Biol Reprod Country: United States |
Other Details:
|
Languages: eng Pagination: 1083-9 Citation Subset: IM |
Affiliation:
|
Laboratory of Animal Reproduction, College of Agriculture, Kinki University, Nara 631-8505, Japan. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Acetylation
/
drug effects Animals Blastocyst / cytology, drug effects, physiology Cell Differentiation / drug effects, physiology Cell Nucleus / chemistry, physiology* Cells, Cultured Embryonic Development / drug effects, physiology* Female Histone Deacetylase Inhibitors Histone Deacetylases / physiology Histones / analysis, metabolism* Hydroxamic Acids / pharmacology Immunohistochemistry Mice Mice, Inbred Strains Nuclear Transfer Techniques* Oocytes / chemistry, cytology, drug effects, physiology* |
| Chemical | |
Reg. No./Substance:
|
0/Histone Deacetylase Inhibitors; 0/Histones; 0/Hydroxamic Acids; 58880-19-6/trichostatin A; EC 3.5.1.98/Histone Deacetylases |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Hypoxia inhibits differentiation of lineage-specific Rcho-1 trophoblast giant cells.
Next Document: Molecular evidence that growth of dominant follicles involves a reduction in follicle-stimulating ho...