Document Detail

Role of hepatic lipase in the uptake and processing of chylomicron remnants in rat liver.
MedLine Citation:
PMID:  8006526     Owner:  NLM     Status:  MEDLINE    
The participation of hepatic lipase in the initial uptake and processing of chylomicron remnants was studied in the isolated, perfused rat liver. Hepatic lipase activity was either reduced by preperfusion of livers with heparin or inhibited with specific rat hepatic lipase antibodies. [3H]palmitate-labeled chylomicron remnants were recirculated through control and treated livers for 15 min; the livers were then flushed, homogenized, and endosome-rich fractions were isolated. Depletion of hepatic lipase activity by both methods reduced the uptake of chylomicron remnants and hydrolysis of their component triglycerides by perfused rat livers, but at the same time significantly increased the rate of endocytosis of those chylomicron remnants taken up. We conclude that hepatic lipase facilitates, but is not essential for, the initial uptake of chylomicron remnants by rat liver. Furthermore, endocytosis of chylomicron remnants does not require binding to hepatic lipase or the associated hydrolysis of remnant lipids.
S Shafi; S E Brady; A Bensadoun; R J Havel
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Publication Detail:
Type:  In Vitro; Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Journal of lipid research     Volume:  35     ISSN:  0022-2275     ISO Abbreviation:  J. Lipid Res.     Publication Date:  1994 Apr 
Date Detail:
Created Date:  1994-07-20     Completed Date:  1994-07-20     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  0376606     Medline TA:  J Lipid Res     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  709-20     Citation Subset:  IM    
Cardiovascular Research Institute, University of California, San Francisco 94143-0130.
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MeSH Terms
Chylomicrons / metabolism*
Endocytosis / physiology
Heparin / pharmacology
Lipase / immunology,  metabolism*
Liver / enzymology,  metabolism*
Rats, Sprague-Dawley
Grant Support
Reg. No./Substance:
0/Chylomicrons; 9005-49-6/Heparin; EC

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