Document Detail


Role of glutathione in the growth of Bradyrhizobium sp. (peanut microsymbiont) under different environmental stresses and in symbiosis with the host plant.
MedLine Citation:
PMID:  16917515     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Glutathione (GSH) plays an important role in the defence of microorganisms and plants against different environmental stresses. To determine the role of GSH under different stresses, such as acid pH, saline shock, and oxidative shock, a GSH-deficient mutant (Bradyrhizobium sp. 6144-S7Z) was obtained by disruption of the gshA gene, which encodes the enzyme gamma-glutamylcysteine synthetase. Growth of the mutant strain was significantly reduced in liquid minimal saline medium, and the GSH content was very low, about 4% of the wild-type level. The defect, caused by disruption of the gshA gene in the growth of mutant strain, cannot be reversed by the addition of GSH (up to 100 micromol/L) to the liquid minimal saline medium, and the endogenous GSH level was approximately the same as that observed without the addition of GSH. In contrast, the wild-type strain increased the GSH content under these conditions. However, the growth of the mutant strain in a rich medium (yeast extract--mannitol) increased, suggesting that at least some but not all of the functions of GSH could be provided by peptides and (or) amino acids. The symbiotic properties of the mutant were similar to those found in the wild-type strain, indicating that the mutation does not affect the ability of the mutant to form effective nodules.
Authors:
Luciano Sobrevals; Peter Müller; Adriana Fabra; Stella Castro
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Canadian journal of microbiology     Volume:  52     ISSN:  0008-4166     ISO Abbreviation:  Can. J. Microbiol.     Publication Date:  2006 Jul 
Date Detail:
Created Date:  2006-08-18     Completed Date:  2006-10-26     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0372707     Medline TA:  Can J Microbiol     Country:  Canada    
Other Details:
Languages:  eng     Pagination:  609-16     Citation Subset:  IM    
Affiliation:
Departamento de Ciencias Naturales, Facultad de Ciencias Exactas, Universidad Nacional de Río Cuarto, Argentina.
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MeSH Terms
Descriptor/Qualifier:
Arachis hypogaea / microbiology*
Bradyrhizobium / genetics,  metabolism*
Glutamate-Cysteine Ligase / metabolism*
Glutathione / genetics,  metabolism*
Hydrogen-Ion Concentration
Oxidative Stress / physiology
Plant Roots / microbiology
Sodium Chloride / pharmacology
Symbiosis / genetics,  physiology*
Chemical
Reg. No./Substance:
70-18-8/Glutathione; 7647-14-5/Sodium Chloride; EC 6.3.2.2/Glutamate-Cysteine Ligase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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