| Role of glutamate dehydrogenase reaction in the control of citrate pool in yeast. | |
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MedLine Citation:
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PMID: 8138009 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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1. Role of NADP-glutamate dehydrogenase in the depletion of citrate was analyzed using permeabilized yeast cells. 2. Citrate was converted to 2-oxoglutarate, which was then metabolized to glutamate by NADP-glutamate dehydrogenase in the presence of ammonium ion. 3. Formation of 2-oxoglutarate plus glutamate was in good agreement with the concentration of citrate decreased. Glutamate formation can be a good indicator of the depletion of citrate, because 70% of the citrate decreased was converted to glutamate. 4. Glycolytic activity was closely correlated with the decrease in citrate under the in situ conditions. 5. NADP-glutamate dehydrogenase increased in anaerobically grown yeast cells. 6. An effective depletion of citrate by increased synthesis of NADP-glutamate dehydrogenase can explain the lowered mechanism of citrate causing glycolytic stimulation under the anaerobic growth conditions of yeast. |
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Authors:
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M Yoshino; K Murakami |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: The International journal of biochemistry Volume: 25 ISSN: 0020-711X ISO Abbreviation: Int. J. Biochem. Publication Date: 1993 Dec |
Date Detail:
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Created Date: 1994-04-22 Completed Date: 1994-04-22 Revised Date: 2000-12-18 |
Medline Journal Info:
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Nlm Unique ID: 0250365 Medline TA: Int J Biochem Country: ENGLAND |
Other Details:
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Languages: eng Pagination: 1723-7 Citation Subset: IM |
Affiliation:
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Department of Genetics, Institute for Developmental Research, Aichi Prefectural Colony, Japan. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Candida
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enzymology,
metabolism* Cell Membrane Permeability / physiology Citrates / metabolism* Citric Acid Glutamate Dehydrogenase (NADP+) / physiology* Glycolysis / physiology* Saccharomyces cerevisiae / enzymology, metabolism* |
| Chemical | |
Reg. No./Substance:
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0/Citrates; 77-92-9/Citric Acid; EC 1.4.1.4/Glutamate Dehydrogenase (NADP+) |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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