Document Detail

Role of glutamate dehydrogenase reaction in the control of citrate pool in yeast.
MedLine Citation:
PMID:  8138009     Owner:  NLM     Status:  MEDLINE    
1. Role of NADP-glutamate dehydrogenase in the depletion of citrate was analyzed using permeabilized yeast cells. 2. Citrate was converted to 2-oxoglutarate, which was then metabolized to glutamate by NADP-glutamate dehydrogenase in the presence of ammonium ion. 3. Formation of 2-oxoglutarate plus glutamate was in good agreement with the concentration of citrate decreased. Glutamate formation can be a good indicator of the depletion of citrate, because 70% of the citrate decreased was converted to glutamate. 4. Glycolytic activity was closely correlated with the decrease in citrate under the in situ conditions. 5. NADP-glutamate dehydrogenase increased in anaerobically grown yeast cells. 6. An effective depletion of citrate by increased synthesis of NADP-glutamate dehydrogenase can explain the lowered mechanism of citrate causing glycolytic stimulation under the anaerobic growth conditions of yeast.
M Yoshino; K Murakami
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  The International journal of biochemistry     Volume:  25     ISSN:  0020-711X     ISO Abbreviation:  Int. J. Biochem.     Publication Date:  1993 Dec 
Date Detail:
Created Date:  1994-04-22     Completed Date:  1994-04-22     Revised Date:  2000-12-18    
Medline Journal Info:
Nlm Unique ID:  0250365     Medline TA:  Int J Biochem     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  1723-7     Citation Subset:  IM    
Department of Genetics, Institute for Developmental Research, Aichi Prefectural Colony, Japan.
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MeSH Terms
Candida / enzymology,  metabolism*
Cell Membrane Permeability / physiology
Citrates / metabolism*
Citric Acid
Glutamate Dehydrogenase (NADP+) / physiology*
Glycolysis / physiology*
Saccharomyces cerevisiae / enzymology,  metabolism*
Reg. No./Substance:
0/Citrates; 77-92-9/Citric Acid; EC Dehydrogenase (NADP+)

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