Document Detail

Role of glucosyltransferase B in interactions of Candida albicans with Streptococcus mutans and with an experimental pellicle on hydroxyapatite surfaces.
MedLine Citation:
PMID:  21803906     Owner:  NLM     Status:  MEDLINE    
Candida albicans and mutans streptococci are frequently detected in dental plaque biofilms from toddlers afflicted with early childhood caries. Glucosyltransferases (Gtfs) secreted by Streptococcus mutans bind to saliva-coated apatite (sHA) and to bacterial surfaces, synthesizing exopolymers in situ, which promote cell clustering and adherence to tooth enamel. We investigated the potential role Gtfs may play in mediating the interactions between C. albicans SC5314 and S. mutans UA159, both with each other and with the sHA surface. GtfB adhered effectively to the C. albicans yeast cell surface in an enzymatically active form, as determined by scintillation spectroscopy and fluorescence imaging. The glucans formed on the yeast cell surface were more susceptible to dextranase than those synthesized in solution or on sHA and bacterial cell surfaces (P < 0.05), indicating an elevated α-1,6-linked glucose content. Fluorescence imaging revealed that larger numbers of S. mutans cells bound to C. albicans cells with glucans present on their surface than to yeast cells without surface glucans (uncoated). The glucans formed in situ also enhanced C. albicans interactions with sHA, as determined by a novel single-cell micromechanical method. Furthermore, the presence of glucan-coated yeast cells significantly increased the accumulation of S. mutans on the sHA surface (versus S. mutans incubated alone or mixed with uncoated C. albicans; P < 0.05). These data reveal a novel cross-kingdom interaction that is mediated by bacterial GtfB, which readily attaches to the yeast cell surface. Surface-bound GtfB promotes the formation of a glucan-rich matrix in situ and may enhance the accumulation of S. mutans on the tooth enamel surface, thereby modulating the development of virulent biofilms.
S Gregoire; J Xiao; B B Silva; I Gonzalez; P S Agidi; M I Klein; K S Ambatipudi; P L Rosalen; R Bauserman; R E Waugh; H Koo
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural     Date:  2011-07-29
Journal Detail:
Title:  Applied and environmental microbiology     Volume:  77     ISSN:  1098-5336     ISO Abbreviation:  Appl. Environ. Microbiol.     Publication Date:  2011 Sep 
Date Detail:
Created Date:  2011-09-09     Completed Date:  2011-12-21     Revised Date:  2013-06-28    
Medline Journal Info:
Nlm Unique ID:  7605801     Medline TA:  Appl Environ Microbiol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  6357-67     Citation Subset:  IM    
Center for Oral Biology, University of Rochester, Rochester, NY 14642, USA.
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MeSH Terms
Candida albicans / chemistry,  physiology*
Cell Adhesion*
Glucans / metabolism
Glucosyltransferases / metabolism*
Microbial Interactions*
Microscopy, Fluorescence
Saliva / microbiology
Spectrum Analysis
Streptococcus mutans / enzymology*,  physiology*
Grant Support
Reg. No./Substance:
0/Glucans; 1306-06-5/Durapatite; EC 2.4.1.-/1,3-alpha-D-glucan synthase; EC 2.4.1.-/Glucosyltransferases

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