Document Detail

Role of gender and estrogen receptors in the rat aorta endothelium-dependent relaxation to red wine polyphenols.
MedLine Citation:
PMID:  19520189     Owner:  NLM     Status:  MEDLINE    
Regular intake of moderate amounts of beverages rich in polyphenols such as red wine is associated with a protective effect on the vascular system, in part, by increasing the endothelial formation of nitric oxide (NO), a major vasoprotective factor. Since estrogens are potent inducers of NO formation and polyphenols have been shown to have phytoestrogen properties, we determined whether estrogen receptors mediate the stimulatory effect of red wine polyphenols (RWPs) on the endothelial formation of NO using isolated rat aortic rings and cultured endothelial cells. RWPs caused endothelium-dependent relaxations, which were more pronounced in the aorta of female than male rats. Increased relaxations were also observed to acetylcholine but not to sodium nitroprusside. Relaxations to RWPs were abolished by nitro l-arginine and MnTMPyP, markedly reduced by polyethyleneglycol-catalase and wortmannin, and not affected by the estrogen antagonist ICI 182,780 in aortic rings from males and females. eNOS expression was higher in aortic sections of female than male rats. RWPs caused the phosphorylation of Akt and eNOS in endothelial cells, which was unaffected by ICI 182,780. Thus, RWPs cause redox-sensitive PI3-kinase/Akt-dependent NO-mediated relaxations, which are more pronounced in the aorta of female than male rats; an effect most likely due to the increased expression level of eNOS rather than activation of estrogen receptors.
Modou Oumy Kane; Eric Anselm; Yanna Dantas Rattmann; Cyril Auger; Valérie B Schini-Kerth
Related Documents :
18292599 - Endothelial s100a1 modulates vascular function via nitric oxide.
19665059 - Enhancement of vascular relaxation in rat aorta by phytoestrogens from curcuma comosa r...
7564339 - Mechanism of acetylcholine-induced constriction enhanced by endothelial removal in isol...
17971759 - endothelium-derived hyperpolarizing factor (edhf): potential involvement in the physiol...
7514359 - Repeated endothelial removal augments intimal thickening and attenuates edrf release.
1953609 - Endothelial dysfunction in hypercholesterolemia is corrected by l-arginine.
15291469 - Vasomodulating potential of mediterranean wild plant extracts.
18707039 - The preventive effect of met-rantes on postoperative intraperitoneal adhesion formation...
10964499 - Syndecan-1 signals independently of beta1 integrins during raji cell spreading.
Publication Detail:
Type:  Journal Article     Date:  2009-06-09
Journal Detail:
Title:  Vascular pharmacology     Volume:  51     ISSN:  1879-3649     ISO Abbreviation:  Vascul. Pharmacol.     Publication Date:    2009 Aug-Sep
Date Detail:
Created Date:  2009-08-11     Completed Date:  2009-10-30     Revised Date:  2009-11-19    
Medline Journal Info:
Nlm Unique ID:  101130615     Medline TA:  Vascul Pharmacol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  140-6     Citation Subset:  IM    
Laboratoire de Pharmacologie et Physiopathologie Cardiovasculaires, UMR CNRS 7175, Faculté de Pharmacie, Université Louis Pasteur de Strasbourg, Illkirch, France.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
1-Phosphatidylinositol 3-Kinase / antagonists & inhibitors
Acetylcholine / pharmacology
Analysis of Variance
Cardiovascular Diseases / prevention & control
Cells, Cultured
Coronary Vessels
Dose-Response Relationship, Drug
Endothelial Cells / physiology
Endothelium, Vascular / physiology*
Estradiol / pharmacology
Estrogen Antagonists / pharmacology
Flavonoids / analysis,  pharmacology*
Free Radical Scavengers / pharmacology
Nitric Oxide / physiology*
Nitric Oxide Donors / pharmacology
Nitric Oxide Synthase Type III / antagonists & inhibitors,  metabolism
Oncogene Protein v-akt / metabolism
Phenols / analysis,  pharmacology*
Rats, Wistar
Receptors, Estrogen / physiology*
Sex Characteristics*
Vasodilation / physiology*
Wine / analysis*
Reg. No./Substance:
0/Estrogen Antagonists; 0/Flavonoids; 0/Free Radical Scavengers; 0/Nitric Oxide Donors; 0/Phenols; 0/Receptors, Estrogen; 0/polyphenols; 10102-43-9/Nitric Oxide; 50-28-2/Estradiol; 51-84-3/Acetylcholine; EC Oxide Synthase Type III; EC 3-Kinase; EC Protein v-akt

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Previous Document:  Exercise increases the phenylephrine effects in isolated portal vein of trained rats.
Next Document:  Role of S114 in the NADH-induced conformational change and catalysis of 3alpha-hydroxysteroid dehydr...