| Role of the gamma-glutamyl cycle in the regulation of amino acid translocation. | |
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MedLine Citation:
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PMID: 2575355 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Amino acid translocation was studied in the mammary gland of lactating rats and in the placenta of pregnant rats. The uptake of amino acids by the mammary gland is maximal on days 10-14 of lactation and is minimal on days 19-21. However, on day 19 maximal uptake can be restored by injection of 1) small amounts of gamma-glutamyl amino acids, 2) 5-oxoproline, and 3) an inhibitor of 5-oxoprolinase. A severe decrease in uptake of amino acids at the peak of lactation is provoked by anthglutin, an inhibitor of gamma-glutamyltranspeptidase (GGT). Simultaneous injection of 5-oxoproline blocks these effects of anthglutin. In pregnant rats, inhibition (79%) of placental GGT activity by acivicin results in a 50% decrease of placental L-[U-14C]-alanine transfer and 70-80% decrease in its incorporation into the placental and fetal proteins. Infusion of 5-oxoproline to mothers previously treated with acivicin restored the L-[U-14C]-alanine transfer. Acivicin or 5-oxoproline did not modify the transfer and metabolism of D-[U14C]glucose by the fetal placental unit. These results show that the gamma-glutamyl cycle should not be considered a mechanism for amino acid transport but rather a generator of extracellular signals, gamma-glutamyl amino acids, that are converted intracellularly to 5-oxoproline, which activates uptake and/or metabolism of amino acids. |
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Authors:
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J R Viña; M Palacin; I R Puertes; R Hernandez; J Viña |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: The American journal of physiology Volume: 257 ISSN: 0002-9513 ISO Abbreviation: Am. J. Physiol. Publication Date: 1989 Dec |
Date Detail:
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Created Date: 1990-02-12 Completed Date: 1990-02-12 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 0370511 Medline TA: Am J Physiol Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: E916-22 Citation Subset: IM |
Affiliation:
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Departamento de Bioquimica y Biologia Molecular, Universidad de Valencia, Spain. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Alanine
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metabolism Amino Acids / metabolism* Animals Biological Transport Female Fetus / metabolism Glucose / metabolism Homeostasis Lactation / metabolism* Maternal-Fetal Exchange Placenta / metabolism* Pregnancy Pregnancy, Animal / metabolism* Rats Rats, Inbred Strains Uterus / metabolism* gamma-Glutamyltransferase / metabolism |
| Chemical | |
Reg. No./Substance:
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0/Amino Acids; 50-99-7/Glucose; 56-41-7/Alanine; EC 2.3.2.2/gamma-Glutamyltransferase |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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