Document Detail


Role of extracellular peroxidase in the superoxide production by wheat root cells.
MedLine Citation:
PMID:  11732329     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Extracellular peroxidase has been shown to contribute to superoxide production in wounded wheat (Triticum aestivum L. cv. Ljuba) root cells. The superoxide-synthesizing system of root cells was considerably inhibited by KCN and NaN3 and activated by MnCl2 and H2O2. Treatment of roots with salicylic acid and a range of di- and tri-carbonic acids (malic, citric, malonic, fumaric, and succinic acids) stimulated superoxide production in both root cells and extracellular solution. The H2O2-stimulated superoxide production in the extracellular solution was much higher when roots were preincubated with salicylic or succinic acid. Exogenous acids enhanced peroxidase activity in the extracellular solution. Pretreatment of root cells with the detergents trypsin and sodium dodecyl sulfate had similar effects on the peroxidase activity. Significant inhibition of both superoxide production and peroxidase activity by diphenylene iodonium suggests that the specificity of the latter as an inhibitor of NADPH oxidase is doubtful. Results obtained indicate that extra-cellular peroxidase is involved in the superoxide production in wheat root cells. The mobile form of peroxidase can be readily secreted to the apoplastic solution and serve as an emergency enzyme involved in plant wound response.
Authors:
F V Minibayeva; L K Gordon; O P Kolesnikov; A V Chasov
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Protoplasma     Volume:  217     ISSN:  0033-183X     ISO Abbreviation:  Protoplasma     Publication Date:  2001  
Date Detail:
Created Date:  2001-12-04     Completed Date:  2002-02-19     Revised Date:  2002-11-01    
Medline Journal Info:
Nlm Unique ID:  9806853     Medline TA:  Protoplasma     Country:  Austria    
Other Details:
Languages:  eng     Pagination:  125-8     Citation Subset:  IM    
Affiliation:
Institute of Biochemistry and Biophysics, Russian Academy of Sciences, Lobachevsky 2/31, Kazan 420503, Russian Federation.
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MeSH Terms
Descriptor/Qualifier:
Adrenochrome / metabolism
Carbonic Acid / chemistry,  pharmacology
Enzyme Inhibitors / pharmacology
Ferrocyanides / pharmacology
NAD / pharmacology
NADP / pharmacology
Onium Compounds / pharmacology
Peroxidase / metabolism*
Plant Roots / cytology,  drug effects,  enzymology,  metabolism*
Superoxides / metabolism*
Triticum / drug effects,  enzymology,  metabolism*
Chemical
Reg. No./Substance:
0/Enzyme Inhibitors; 0/Ferrocyanides; 0/Onium Compounds; 11062-77-4/Superoxides; 13943-58-3/potassium ferrocyanide; 244-54-2/diphenyleneiodonium; 463-79-6/Carbonic Acid; 53-59-8/NADP; 53-84-9/NAD; 54-06-8/Adrenochrome; EC 1.11.1.7/Peroxidase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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