Document Detail

Role of extracellular matrix in regulating fenestrations of sinusoidal endothelial cells isolated from normal rat liver.
MedLine Citation:
PMID:  1592359     Owner:  NLM     Status:  MEDLINE    
Open fenestrations are a conspicuous feature of sinusoidal endothelial cells and allow free movement of plasma into the space of Disse. In hepatic fibrosis, the number of fenestrations decreases as interstitial collagen increases in the liver, a change that correlates with deposition of extracellular matrix in the space of Disse. In this study, the possibility of a causal relationship between altered fenestral morphology and perisinusoidal matrix has been examined by culturing rat sinusoidal endothelial cells on individual matrix proteins or on a native matrix consisting of human amniotic membrane with interstitial collagen (types I and III) on one side and basement membrane proteins (collagen types IV and V and laminin) on the other. Under culture conditions, individual components of the extracellular matrix failed to maintain fenestrations. A basement-membranelike gel matrix derived from the Engelbreth-Holm-Swarm tumor war similarly ineffective. Fenestral density and porosity (percentage of cell surface occupied by fenestrations) were significantly enhanced, however, when endothelial cells were cultured on the basement-membrane side of human amnion. These data suggest that support of endothelial fenestrations requires a complex matrix. In particular, physiologically derived basement membrane maintains fenestrations, whereas interstitial collagen matrix does not. The loss of fenestrations associated with hepatic fibrosis may be related in part to an accumulation of interstitial collagens in the space of Disse.
R F McGuire; D M Bissell; J Boyles; F J Roll
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Hepatology (Baltimore, Md.)     Volume:  15     ISSN:  0270-9139     ISO Abbreviation:  Hepatology     Publication Date:  1992 Jun 
Date Detail:
Created Date:  1992-06-26     Completed Date:  1992-06-26     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  8302946     Medline TA:  Hepatology     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  989-97     Citation Subset:  IM    
Liver Center, San Francisco General Hospital, University of California, San Francisco 94110.
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MeSH Terms
Basement Membrane
Cells, Cultured
Collagen / metabolism
Endothelium, Vascular / cytology*
Extracellular Matrix / metabolism,  physiology*
Liver / blood supply*,  cytology
Microscopy, Electron, Scanning
Rats, Inbred Strains
Grant Support
Reg. No./Substance:

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