| Role of extracellular UTP in the release of uracil from vasoconstricted hindlimb. | |
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MedLine Citation:
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PMID: 8430851 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The source and function of elevated uracil release during vasoconstriction in the perfused rat hindlimb was investigated. The possibility that uracil release derived from the breakdown of released vasoactive uridine 5'-triphosphate (UTP) was examined. Exogenous UTP was found to be a potent vasodilator in the perfused rat hindlimb, opposing norepinephrine and angiotensin-induced increases in vasoconstriction and oxygen consumption. UTP was rapidly catabolized by the hindlimb to uridine 5'-monophosphate (UMP), uridine, and uracil, which were all devoid of vasoactivity. UTP was similarly catabolized by incubated rat aorta. Degradation of exogenous UTP by perfused hindlimb or aorta was inhibited by alpha, beta-methylene-adenosine 5'-diphosphate (AMP-CP), an inhibitor of ectonucleotidases. However, AMP-CP did not decrease uracil and uridine output by the hindlimb during angiotensin-mediated vasoconstriction and increased oxygen consumption. In particular, simultaneous infusion of AMP-CP with angiotensin did not increase efflux of UMP. Although exogenous UTP is a potent vasodilator in the perfused rat hindlimb, it appears not to be released intact during vasoconstriction. Hence, extracellular UTP is unlikely to be the precursor of the uracil release. |
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Authors:
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S M Richards; K A Dora; S Rattigan; E Q Colquhoun; M G Clark |
Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: The American journal of physiology Volume: 264 ISSN: 0002-9513 ISO Abbreviation: Am. J. Physiol. Publication Date: 1993 Jan |
Date Detail:
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Created Date: 1993-03-05 Completed Date: 1993-03-05 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 0370511 Medline TA: Am J Physiol Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: H233-7 Citation Subset: IM |
Affiliation:
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Department of Biochemistry, University of Tasmania, Hobart, Australia. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Adenosine Diphosphate
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pharmacology Adenosine Triphosphate / pharmacology Animals Aorta / metabolism Blood Pressure / drug effects Extracellular Space / metabolism* Hindlimb / blood supply Male Muscle, Smooth, Vascular / metabolism* Nucleotides / metabolism Oxygen Consumption / drug effects Rats Rats, Wistar Uracil / metabolism* Uridine Monophosphate / antagonists & inhibitors, metabolism Uridine Triphosphate / metabolism*, pharmacology Vasoconstriction* |
| Chemical | |
Reg. No./Substance:
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0/Nucleotides; 3805-37-6/adenosine 2',5'-diphosphate; 56-65-5/Adenosine Triphosphate; 58-64-0/Adenosine Diphosphate; 58-97-9/Uridine Monophosphate; 63-39-8/Uridine Triphosphate; 66-22-8/Uracil |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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