Document Detail

Role of Na+/H+ exchanger in resveratrol-induced growth inhibition of human breast cancer cells.
MedLine Citation:
PMID:  21191669     Owner:  NLM     Status:  MEDLINE    
Cancer cells maintain low intracellular pH [pHi]; therefore, it is likely that resveratrol [RSVL] inhibits cell growth through interference with regulation of pHi. Na-H exchanger [NHE] regulates pHi and NaCl uptake. In this study, we investigated a putative role of NHE-1 and NHE-3 isoforms in the RSVL-induced cell death using MDA-MB-231 estrogen receptor-negative [ER-] and MCF-7 [ER+] human breast cancer cell lines. ECL Western blot analysis and fluorescence morphometeric analysis were used. Cell viability and counting were performed using standard procedures. RSVL caused a dose- and time-dependent induction of NHE-1 and NHE-3 proteins in both cancer cell lines as shown by ECL Western blot analysis and fluorescence measurement. Interestingly, the level of actin, an internal control, remains unaltered. Thus, it is concluded that RSVL-inhibited cell growth and viability, increased cell size, and volume along with an increased apoptotic activity are due to the induction of NHE-1 and NHE-3 isoforms in the present breast cancer cell lines. Induction of NHE will increase the uptake of NaCl and decrease pHi leading to disturbance in Ca(2+) homeostasis, which is responsible for cell death.
Heba Mehdawi; Moussa Alkhalaf; Islam Khan
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2010-12-30
Journal Detail:
Title:  Medical oncology (Northwood, London, England)     Volume:  29     ISSN:  1559-131X     ISO Abbreviation:  Med. Oncol.     Publication Date:  2012 Mar 
Date Detail:
Created Date:  2012-02-09     Completed Date:  2012-08-23     Revised Date:  2013-06-03    
Medline Journal Info:
Nlm Unique ID:  9435512     Medline TA:  Med Oncol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  25-32     Citation Subset:  IM    
Department of Biochemistry, Faculty of Medicine, Kuwait University, PO Box 24923, Kuwait City, Kuwait.
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MeSH Terms
Antineoplastic Agents, Phytogenic / pharmacology*
Apoptosis / drug effects
Blotting, Western
Breast Neoplasms / metabolism*
Cation Transport Proteins / biosynthesis*
Cell Line, Tumor
Cell Proliferation / drug effects*
Cell Survival / drug effects
Fluorescent Antibody Technique
Microscopy, Confocal
Sodium-Hydrogen Antiporter / biosynthesis*
Stilbenes / pharmacology*
Reg. No./Substance:
0/Antineoplastic Agents, Phytogenic; 0/Cation Transport Proteins; 0/SLC9A1 protein, human; 0/Sodium-Hydrogen Antiporter; 0/Stilbenes; 0/sodium-hydrogen exchanger 3; Q369O8926L/resveratrol

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