Document Detail


Role of the evolutionarily conserved cytochrome b tryptophan 142 in the ubiquinol oxidation catalyzed by the bc1 complex in the yeast Saccharomyces cerevisiae.
MedLine Citation:
PMID:  7673215     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Trp-142 is a highly conserved residue of the cytochrome b subunit in the bc1 complexes. To study the importance of this residue in the quinol oxidation catalyzed by the bc1 complex, we characterized four yeast mutants with arginine, lysine, threonine, and serine at position 142. The mutant W142R was isolated previously as a respiration-deficient mutant unable to grow on non-fermentable carbon sources (Lemesle-Meunier, D., Brivet-Chevillotte, P., di Rago, J.-P, Slonimski, P.P., Bruel, C., Tron, T., and Forget, N. (1993) J. Biol. Chem. 268, 15626-15632). The mutants W142K, W142T, and W142S were obtained here as respiration-sufficient revertants from mutant W142R. Mutant W142R exhibited a decreased complex II turnover both in the presence and absence of antimycin A; this suggests that the structural effect of W142R in the bc1 complex probably interferes with the correct assembly of the succinate-ubiquinone reductase complex. The mutations resulted in a parallel decrease in turnover number and apparent Km, with the result that there was no significant change in the second-order rate constant for ubiquinol oxidation. Mutants W142K and W142T exhibited some resistance toward myxothiazol, whereas mutant W142R showed increased sensitivity. The cytochrome cc1 reduction kinetics were found to be severely affected in mutants W142R, W142K, and W142T. The respiratory activities and the amounts of reduced cytochrome b measured during steady state suggest that the W142S mutation also modified the quinol-cytochrome c1 electron transfer pathway. The cytochrome b reduction kinetics through center P were affected when Trp-142 was replaced with arginine or lysine, but not when it was replaced with threonine or serine. Of the four amino acids tested at position 142, only arginine resulted in a decrease in cytochrome b reduction through center N. These findings are discussed in terms of the structure and function of the quinol oxidation site and seem to indicate that Trp-142 is not critical to the kinetic interaction of ubiquinol with the reductase, but plays an important role in the electron transfer reactions that intervene between ubiquinol oxidation and cytochrome c1 reduction.
Authors:
C Bruel; J P di Rago; P P Slonimski; D Lemesle-Meunier
Related Documents :
17560895 - Two-dimensional infrared correlation spectroscopy study of the interaction of oxidized ...
2868895 - Rhodamine 6g inhibits the matrix-catalyzed processing of precursors of rat-liver mitoch...
18259985 - Cytochromes p450: a structure-based summary of biotransformations using representative ...
17625855 - Modulation of heme redox potential in the cytochrome c6 family.
16460035 - The kinase homology domain of receptor guanylyl cyclase c: atp binding and identificati...
17215875 - Erp57 and pdi: multifunctional protein disulfide isomerases with similar domain archite...
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  The Journal of biological chemistry     Volume:  270     ISSN:  0021-9258     ISO Abbreviation:  J. Biol. Chem.     Publication Date:  1995 Sep 
Date Detail:
Created Date:  1995-10-17     Completed Date:  1995-10-17     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  2985121R     Medline TA:  J Biol Chem     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  22321-8     Citation Subset:  IM    
Affiliation:
Laboratoire de Bioénergétique et Ingénierie des Protéines, CNRS, Marseille, France.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:
Amino Acid Sequence
Binding Sites
Electron Transport
Electron Transport Complex III / chemistry,  metabolism*
Evolution
Mitochondria / metabolism
Molecular Sequence Data
Point Mutation
Protein Structure, Secondary
Saccharomyces cerevisiae / enzymology*
Spectrum Analysis
Structure-Activity Relationship
Tryptophan
Ubiquinone / analogs & derivatives*,  metabolism
Chemical
Reg. No./Substance:
1339-63-5/Ubiquinone; 56275-39-9/ubiquinol; 73-22-3/Tryptophan; EC 1.10.2.2/Electron Transport Complex III

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Vesicular L-glutamate transporter in microvesicles from bovine pineal glands. Driving force, mechani...
Next Document:  Vma22p is a novel endoplasmic reticulum-associated protein required for assembly of the yeast vacuol...