Document Detail

Role of endothelium-derived prostaglandins in hypoxia-elicited arteriolar dilation in rat skeletal muscle.
MedLine Citation:
PMID:  1516156     Owner:  NLM     Status:  MEDLINE    
The aims of the present study were to determine the response of rat cremaster muscle first-order arterioles to hypoxia and the role of endothelium-derived prostaglandins in the response. Isolated arterioles were cannulated, pressurized to 65 mm Hg, and studied in a no-flow condition in a bath containing Krebs' bicarbonate solution, pH 7.4, equilibrated with 21% O2-5% CO2-74% N2 (PO2, 150 mm Hg) or 95% N2-5% CO2 (PO2, 15 mm Hg [hypoxia]). Responses to hypoxia and vasoactive substances were studied before and after removal of the endothelium or blockade of prostaglandin synthesis by the administration of indomethacin (10(-5) M). Addition to the suffusion solution of arachidonic acid (10(-7) and 10(-6) M), prostaglandin E2 (10(-9) and 10(-8) M), acetylcholine (10(-8) and 10(-6) M), or sodium nitroprusside (10(-8) M) evoked significant arteriolar dilation. When the bath PO2 was reduced from 150 to 15 mm Hg, arteriolar diameters increased by 58.8 +/- 9.3 microns (61%). Removal of the endothelium completely inhibited responses to hypoxia, acetylcholine, and arachidonic acid, whereas responses to sodium nitroprusside and prostaglandin E2 remained unaltered. In arterioles with an intact endothelium, indomethacin completely inhibited the responses to hypoxia and arachidonic acid, whereas responses to acetylcholine and sodium nitroprusside were unaltered. These findings support the conclusion that endothelium-derived prostaglandins mediate the arteriolar dilation to hypoxia in rat skeletal muscle arterioles.
E J Messina; D Sun; A Koller; M S Wolin; G Kaley
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Publication Detail:
Type:  Comparative Study; In Vitro; Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Circulation research     Volume:  71     ISSN:  0009-7330     ISO Abbreviation:  Circ. Res.     Publication Date:  1992 Oct 
Date Detail:
Created Date:  1992-10-08     Completed Date:  1992-10-08     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  0047103     Medline TA:  Circ Res     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  790-6     Citation Subset:  IM    
Department of Physiology, New York Medical College, Valhalla 10595.
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MeSH Terms
Acetylcholine / pharmacology
Anoxia / physiopathology*
Arachidonic Acid / pharmacology
Arterioles / drug effects*,  physiology
Dinoprostone / pharmacology
Endothelium, Vascular / drug effects,  physiology*
Indomethacin / pharmacology
Muscle, Smooth, Vascular / drug effects,  physiology
Muscles / blood supply*
Nitroprusside / pharmacology
Oxygen / administration & dosage
Prostaglandins / biosynthesis,  physiology*
Rats, Inbred Strains
Vasodilation* / drug effects
Grant Support
Reg. No./Substance:
0/Prostaglandins; 15078-28-1/Nitroprusside; 363-24-6/Dinoprostone; 506-32-1/Arachidonic Acid; 51-84-3/Acetylcholine; 53-86-1/Indomethacin; 7782-44-7/Oxygen

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