Document Detail

Role for the endometrial epithelial protein MFG-E8 and its receptor integrin αvβ3 in human implantation: results of an in vitro trophoblast attachment study using established human cell lines.
MedLine Citation:
PMID:  24424369     Owner:  NLM     Status:  Publisher    
OBJECTIVE: To investigate the role of MFG-E8 and its receptor integrin αvβ3 in the attachment of trophoblast cells to the endometrial epithelium.
DESIGN: Experimental in vitro study.
SETTING: Academic center.
INTERVENTION(S): By using a well-differentiated endometrial adenocarcinoma cell line (Ishikawa cells) and choriocarcinoma human trophoblast cells (Jar cells), an in vitro assay mimicking human implantation was established. To investigate the impact of blocking MFG-E8 and integrin αvβ3, we pretreated the cell lines with antibodies against those proteins at different concentrations before the attachment assay.
MAIN OUTCOME MEASURE(S): Attachment rate of Jar spheroids to the epithelial cell monolayer.
RESULT(S): Pretreatment of Ishikawa cells with anti-MFG-E8 antibody caused a dose-dependent and significant inhibition of attachment. On the other hand, pretreatment of Jar spheroids did not result in a significant effect on the attachment rate. Pretreatment of Ishikawa cells as well as Jar spheroids with anti-integrin αvβ3 antibodies resulted in a dose-dependent, significant inhibition of attachment.
CONCLUSION(S): This study showed that blocking MFG-E8 and its receptor integrin αvβ3 in Ishikawa cells diminishes Jar spheroid attachment. Moreover, blocking integrin αvβ3 in the trophoblastic cells also diminished their attachment to the Ishikawa monolayer.
Carla Schmitz; Liang Yu; Silvina Bocca; Sandra Anderson; João Sabino Cunha-Filho; Bhaskara S Rhavi; Sergio Oehninger
Related Documents :
24460329 - Mir-21 upregulation induced by promoter zone histone acetylation is associated with che...
24979279 - Selective creb-dependent cyclin expression mediated by the pi3k and mapk pathways suppo...
23382939 - Metabolites of ginger component [6]-shogaol remain bioactive in cancer cells and have l...
23121049 - Functional gpr37 trafficking protects against toxicity induced by 6-ohda, mpp+ or roten...
24410759 - Hmgb1 accelerates early acute allograft rejection via enhancing il-17(+) γδ t cells res...
24444609 - Restoration of p53/mir-34a regulatory axis decreases survival advantage and ensures bax...
16216929 - Inhibition of cell movement and proliferation by cell-cell contact-induced interaction ...
11239649 - Aspirin effects on endometrial cancer cell growth.
17879239 - Cytometric assessment of dna damage by exogenous and endogenous oxidants reports aging-...
Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2014-1-11
Journal Detail:
Title:  Fertility and sterility     Volume:  -     ISSN:  1556-5653     ISO Abbreviation:  Fertil. Steril.     Publication Date:  2014 Jan 
Date Detail:
Created Date:  2014-1-15     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0372772     Medline TA:  Fertil Steril     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Copyright Information:
Copyright © 2014 American Society for Reproductive Medicine. Published by Elsevier Inc. All rights reserved.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Previous Document:  Hyperandrogenemia is implicated in both the metabolic and reproductive morbidities of polycystic ova...
Next Document:  Validation of the power model of ovarian nongrowing follicle depletion associated with aging in wome...