Document Detail


Role of endogenous hydrogen sulfide in neurogenic relaxation of rat corpus cavernosum.
MedLine Citation:
PMID:  22306852     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Relaxation of corpus cavernosum during penile erection is mediated by a non-adrenergic non-cholinergic (NANC) neurotransmission and by the endothelium via the release of nitric oxide. Hydrogen sulfide (H(2)S) is an endogenous gaseous mediator which is a potent vasodilator and a neurotransmitter. This study was initiated to characterize the role of H(2)S in NANC neurogenic transmission in rat corpus cavernosum. The expression of H(2)S producing enzymes was assessed using RT-PCR as well as Western blotting and showed the expression of cystathionine γ-lyase (CSE) in rat corporal tissue. Homogenates from rat corpus cavernosum convert l-cysteine to H(2)S and this was partially inhibited by a CSE inhibitor, propargylglycine. Electrical stimulation of corporal tissue strips caused NANC relaxation. This neurogenic relaxation was significantly enhanced by inhibition of CSE by propargylglycine indicating that endogenously produced H(2)S may have a negative regulatory role in neurogenic relaxation of rat corpus cavernosum. To investigate this further we used physiologically relevant concentrations of exogenous NaHS, and showed that nanomolar concentrations could inhibit corporal relaxation induced by a nitroxyl (HNO) donor (Angeli's salt) but not with nitrosonium (NO(+)) or NO donors. This suggests that an interaction between endogenously produced H(2)S and nitroxyl (HNO) might be involved in erectile function.
Authors:
Mehdi Ghasemi; Ahmad R Dehpour; Kevin P Moore; Ali R Mani
Publication Detail:
Type:  In Vitro; Journal Article; Research Support, Non-U.S. Gov't     Date:  2012-01-28
Journal Detail:
Title:  Biochemical pharmacology     Volume:  83     ISSN:  1873-2968     ISO Abbreviation:  Biochem. Pharmacol.     Publication Date:  2012 May 
Date Detail:
Created Date:  2012-03-12     Completed Date:  2012-05-21     Revised Date:  2014-02-20    
Medline Journal Info:
Nlm Unique ID:  0101032     Medline TA:  Biochem Pharmacol     Country:  England    
Other Details:
Languages:  eng     Pagination:  1261-8     Citation Subset:  IM    
Copyright Information:
Copyright © 2012 Elsevier Inc. All rights reserved.
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MeSH Terms
Descriptor/Qualifier:
Alkynes / pharmacology
Animals
Cystathionine beta-Synthase / genetics,  metabolism
Cystathionine gamma-Lyase / antagonists & inhibitors,  genetics,  metabolism
Dose-Response Relationship, Drug
Glycine / analogs & derivatives,  pharmacology
Hydrogen Sulfide / metabolism*,  pharmacology
Male
Muscle Relaxation / physiology*
Nitrogen Oxides / pharmacology
Penile Erection / physiology
Penis / physiology*
Rats
Rats, Sprague-Dawley
Grant Support
ID/Acronym/Agency:
G0800971//Medical Research Council
Chemical
Reg. No./Substance:
0/Alkynes; 0/Nitrogen Oxides; 14332-28-6/nitroxyl; 64165-64-6/propargylglycine; EC 4.2.1.22/Cystathionine beta-Synthase; EC 4.4.1.1/Cystathionine gamma-Lyase; TE7660XO1C/Glycine; YY9FVM7NSN/Hydrogen Sulfide

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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