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Role of elovl4 protein in the biosynthesis of docosahexaenoic Acid.
MedLine Citation:
PMID:  20238022     Owner:  NLM     Status:  In-Data-Review    
Abstract/OtherAbstract:
The disk membranes of retinal photoreceptor outer segments and other neuronal and reproductive tissues are enriched in docosahexaenoic acid (DHA, 22:6n3), which is essential for their normal function and development. The fatty acid condensing enzyme Elongation of Very Long chain fatty acids-4 (ELOVL4) is highly expressed in retina photoreceptors as well as other tissues with high 22:6n3 content. Mutations in the ELOVL4 gene are associated with autosomal dominant Stargardt-like macular dystrophy (STGD3) and results in synthesis of a truncated protein that cannot be targeted to the endoplasmic reticulum (ER), the site of fatty acid biosynthesis. Considering the abundance and essential roles of 22:6n3 in ELOVL4-expressing tissues (except the skin), it was proposed that the ELOVL4 protein may be involved in 22:6n3 biosynthesis. We tested the hypothesis that the ELOVL4 protein is involved in 22:6n3 biosynthesis by selectively silencing expression of the protein in the cone photoreceptors derived cell line 661 w and showed that the ELOVL4 protein is not involved in DHA biosynthesis from the short chain fatty acid precursors 18:3n3 and 22:5n3.
Authors:
Martin-Paul Agbaga; Richard S Brush; Md Nawajes A Mandal; Michael H Elliott; Muayyad R Al-Ubaidi; Robert E Anderson
Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Advances in experimental medicine and biology     Volume:  664     ISSN:  0065-2598     ISO Abbreviation:  Adv. Exp. Med. Biol.     Publication Date:  2010  
Date Detail:
Created Date:  2010-03-18     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0121103     Medline TA:  Adv Exp Med Biol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  233-42     Citation Subset:  IM    
Affiliation:
Department of Cell Biology, Dean McGee Eye Institute, University of Oklahoma Health Sciences Center, 608 Stanton L.Young Blvd, DMEI 409, Oklahoma, OK, 73104, USA, martin-paul-agbaga@ouhsc.edu.
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