Document Detail


Role of Elovl4 protein in the biosynthesis of docosahexaenoic acid.
MedLine Citation:
PMID:  20238022     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The disk membranes of retinal photoreceptor outer segments and other neuronal and reproductive tissues are enriched in docosahexaenoic acid (DHA, 22:6n3), which is essential for their normal function and development. The fatty acid condensing enzyme Elongation of Very Long chain fatty acids-4 (ELOVL4) is highly expressed in retina photoreceptors as well as other tissues with high 22:6n3 content. Mutations in the ELOVL4 gene are associated with autosomal dominant Stargardt-like macular dystrophy (STGD3) and results in synthesis of a truncated protein that cannot be targeted to the endoplasmic reticulum (ER), the site of fatty acid biosynthesis. Considering the abundance and essential roles of 22:6n3 in ELOVL4-expressing tissues (except the skin), it was proposed that the ELOVL4 protein may be involved in 22:6n3 biosynthesis. We tested the hypothesis that the ELOVL4 protein is involved in 22:6n3 biosynthesis by selectively silencing expression of the protein in the cone photoreceptors derived cell line 661 w and showed that the ELOVL4 protein is not involved in DHA biosynthesis from the short chain fatty acid precursors 18:3n3 and 22:5n3.
Authors:
Martin-Paul Agbaga; Richard S Brush; Md Nawajes A Mandal; Michael H Elliott; Muayyad R Al-Ubaidi; Robert E Anderson
Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Advances in experimental medicine and biology     Volume:  664     ISSN:  0065-2598     ISO Abbreviation:  Adv. Exp. Med. Biol.     Publication Date:  2010  
Date Detail:
Created Date:  2010-03-18     Completed Date:  2011-09-12     Revised Date:  2014-09-15    
Medline Journal Info:
Nlm Unique ID:  0121103     Medline TA:  Adv Exp Med Biol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  233-42     Citation Subset:  IM    
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:
Animals
Cell Line
Docosahexaenoic Acids / biosynthesis*
Eye Proteins / genetics,  metabolism*
Gene Expression Regulation
Gene Knockdown Techniques
Membrane Proteins / genetics,  metabolism*
Mice
RNA, Messenger / genetics,  metabolism
RNA, Small Interfering / metabolism
Grant Support
ID/Acronym/Agency:
EY00871/EY/NEI NIH HHS; EY04149/EY/NEI NIH HHS; EY12190/EY/NEI NIH HHS; R01 EY004149/EY/NEI NIH HHS; R01EY14052/EY/NEI NIH HHS; RR17703/RR/NCRR NIH HHS
Chemical
Reg. No./Substance:
0/Elovl4 protein, mouse; 0/Eye Proteins; 0/Membrane Proteins; 0/RNA, Messenger; 0/RNA, Small Interfering; 25167-62-8/Docosahexaenoic Acids

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Photoreceptor sensory cilia and inherited retinal degeneration.
Next Document:  Molecular pathogenesis of achromatopsia associated with mutations in the cone cyclic nucleotide-gate...