Document Detail


Role of the effect of inhibition of neutral endopeptidase on vascular and neural complications in streptozotocin-induced diabetic rats.
MedLine Citation:
PMID:  21040718     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
We have previously shown that treating streptozotocin-induced diabetic rats, an animal model of type 1 diabetes, with Ilepatril (an inhibitor of neutral endopeptidase and angiotensin converting enzyme (ACE)) improves vascular and neural function. In this study we sought to determine the individual effect of inhibition of neutral endopeptidase and ACE on diabetes-induced vascular and neural dysfunction. After 4 weeks of untreated diabetes, rats were treated for 12 weeks with Ilepatril, Enalapril (ACE inhibitor) or Candoxatril (neutral endopeptidase inhibitor) followed by analysis of neural and vascular function. Diabetes caused slowing of motor and sensory nerve conduction, thermal hypoalgesia, reduction in intraepidermal nerve fiber density in the hindpaw and impairment in vascular relaxation to acetylcholine and calcitonin gene-related peptide in epineural arterioles of the sciatic nerve and to atrial natriuretic peptide and calcitonin gene-related peptide in renal arteries. Inhibition of neutral endopeptidase or ACE improved neural function; however, dual inhibition of neutral endopeptidase and ACE with Ilepatril tended to have the greatest efficacy. Ilepatril and Candoxatril treatment of diabetic rats was more efficacious in improving vascular responsiveness in epineurial arterioles than treatment with Enalapril. Ilepatril, Enalapril or Candoxatril treatment of diabetic rats were all efficacious in renal arteries. These studies suggest that combination therapy may be the most effective approach for treatment of diabetic neural and vascular complications.
Authors:
Christine L Oltman; Eric P Davidson; Lawrence J Coppey; Travis L Kleinschmidt; Brian Dake; Mark A Yorek
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Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S.     Date:  2010-10-30
Journal Detail:
Title:  European journal of pharmacology     Volume:  650     ISSN:  1879-0712     ISO Abbreviation:  Eur. J. Pharmacol.     Publication Date:  2011 Jan 
Date Detail:
Created Date:  2010-12-14     Completed Date:  2011-05-02     Revised Date:  2012-03-07    
Medline Journal Info:
Nlm Unique ID:  1254354     Medline TA:  Eur J Pharmacol     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  556-62     Citation Subset:  IM    
Copyright Information:
Published by Elsevier B.V.
Affiliation:
Department of Veterans Affairs Iowa City Health Care System, University of Iowa, Iowa City, IA 52246, USA.
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MeSH Terms
Descriptor/Qualifier:
Angiotensin-Converting Enzyme Inhibitors / pharmacology
Animals
Diabetes Mellitus, Experimental / complications,  drug therapy*,  physiopathology
Diabetic Angiopathies / drug therapy,  etiology,  physiopathology
Diabetic Neuropathies / drug therapy,  etiology,  physiopathology
Enalapril / pharmacology*,  therapeutic use
Heterocyclic Compounds, 3-Ring / pharmacology*,  therapeutic use
Indans / pharmacology*,  therapeutic use
Male
Neprilysin / antagonists & inhibitors*
Neural Conduction / drug effects*
Propionates / pharmacology*,  therapeutic use
Rats
Rats, Sprague-Dawley
Sciatic Nerve / drug effects,  physiopathology
Streptozocin
Grant Support
ID/Acronym/Agency:
DK073990/DK/NIDDK NIH HHS; R01 DK073990-03/DK/NIDDK NIH HHS; R01 DK073990-05/DK/NIDDK NIH HHS
Chemical
Reg. No./Substance:
0/AVE 7688; 0/Angiotensin-Converting Enzyme Inhibitors; 0/Heterocyclic Compounds, 3-Ring; 0/Indans; 0/Propionates; 118785-03-8/candoxatril; 18883-66-4/Streptozocin; 75847-73-3/Enalapril; EC 3.4.24.11/Neprilysin

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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