Document Detail


Role of eIF3a in regulating cell cycle progression.
MedLine Citation:
PMID:  19327350     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Translational control is an essential process in regulation of gene expression, which occurs at the initiation step performed by a number of translation initiation factor complexes. eIF3a (eIF3 p170) is the largest subunit of the eIF3 complex. eIF3a has been suggested to play roles in regulating translation of a subset of mRNAs and in regulating cell cycle progression and cell proliferation. In this study, we examined the expression profile of eIF3a in cell cycle and its role in cell cycle progression. We found that eIF3a expression oscillated with cell cycle and peaked in S phase. Reducing eIF3a expression also reduced cell proliferation rate by elongating cell cycle but did not change the cell cycle distribution. However, eIF3a appears to play an important role in cellular responses to external cell cycle modulators likely by affecting synthesis of target proteins of these modulators.
Authors:
Zizheng Dong; Zhaoqian Liu; Ping Cui; Roxana Pincheira; Youyun Yang; Jianguo Liu; Jian-Ting Zhang
Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural     Date:  2009-03-24
Journal Detail:
Title:  Experimental cell research     Volume:  315     ISSN:  1090-2422     ISO Abbreviation:  Exp. Cell Res.     Publication Date:  2009 Jul 
Date Detail:
Created Date:  2009-06-01     Completed Date:  2009-06-16     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0373226     Medline TA:  Exp Cell Res     Country:  United States    
Other Details:
Languages:  eng     Pagination:  1889-94     Citation Subset:  IM    
Affiliation:
Department of Pharmacology and Toxicology, IU Simon Cancer Center, Walther Oncology Center/Walther Cancer Institute, Indiana University School of Medicine, Indianapolis, IN 46202, USA.
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MeSH Terms
Descriptor/Qualifier:
Animals
Base Sequence
Cell Cycle / genetics,  physiology*
Cell Line
Cell Proliferation
DNA Primers / genetics
DNA, Antisense / genetics
Down-Regulation
Eukaryotic Initiation Factor-3 / antagonists & inhibitors,  genetics,  physiology*
Gene Expression
Humans
Mice
NIH 3T3 Cells
Protein Biosynthesis
RNA, Messenger / genetics,  metabolism
Grant Support
ID/Acronym/Agency:
CA94961/CA/NCI NIH HHS
Chemical
Reg. No./Substance:
0/DNA Primers; 0/DNA, Antisense; 0/Eukaryotic Initiation Factor-3; 0/RNA, Messenger

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