Document Detail


Role of divalent cations, pH, cytoskeleton components and surface charge on the adhesion of Trichomonas vaginalis to a polystyrene substrate.
MedLine Citation:
PMID:  3509860     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The process of adhesion of three different strains of Trichomonas vaginalis to a polystyrene substrate was analysed. The process of adhesion was dependent on the time of incubation and the pH of the phosphate-buffered solution (PBS) in which the parasites were suspended. The highest indices of adhesion were observed after an incubation time of 60 min at pH 6.6. The adhesion index increased when the parasites were incubated in the presence of culture media or when Ca++ or Mg++ was added to the PBS solution, whereas cytochalasin B, trypsin or neuraminidase reduced adhesion. Incubation of the parasites in the presence of poly-L-lysine facilitated the process of adhesion. Incubation of the parasites or polystyrene beads in the presence of poly-L-lysine led to important changes in their surface charge.
Authors:
F C Silva Filho; C A Elias; W De Souza
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Memórias do Instituto Oswaldo Cruz     Volume:  82     ISSN:  0074-0276     ISO Abbreviation:  Mem. Inst. Oswaldo Cruz     Publication Date:    1987 Jul-Sep
Date Detail:
Created Date:  1989-05-05     Completed Date:  1989-05-05     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  7502619     Medline TA:  Mem Inst Oswaldo Cruz     Country:  BRAZIL    
Other Details:
Languages:  eng     Pagination:  379-84     Citation Subset:  IM    
Affiliation:
Instituto Oswaldo Cruz, Departamento de Protozoologia, Rio de Janeiro, Brasil.
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MeSH Terms
Descriptor/Qualifier:
Animals
Calcium / physiology
Cell Adhesion
Culture Media
Cytoskeleton / physiology
Hydrogen-Ion Concentration
Magnesium / physiology
Neuraminidase / metabolism*
Polystyrenes / metabolism*
Trichomonas vaginalis / physiology*,  ultrastructure
Chemical
Reg. No./Substance:
0/Culture Media; 0/Polystyrenes; 7439-95-4/Magnesium; 7440-70-2/Calcium; EC 3.2.1.18/Neuraminidase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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