Document Detail


Role of cytosolic phospholipase A2 in cytokine-stimulated prostaglandin release by human gallbladder cells.
MedLine Citation:
PMID:  10675243     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Eicosanoids are involved in gallbladder inflammation, epithelial water transport, and mucous secretion. Phospholipase Asubscript2 enzymes liberate arachidonic acid from membrane phospholipids for the synthesis of eicosanoids. The purpose of this study was to determine the effect of selective cytoplasmic and secretory phospholipase A2 inhibitors on basal and stimulated arachidonic acid and prostaglandin E2 release in gallbladder cells. Western immunoblotting was employed to evaluate both cytosolic and secretory phospholipase A2 enzymes in human gallbladder cells. Cells were incubated for 22 hours with (3)H-labeled arachidonic acid. Arachidonic acid and prostaglandin E2 release was then measured in the supernate after 2 hours of exposure to human interleukin-1beta, alone or after pretreatment for 1 hour with the inhibitors. Unstimulated gallbladder cells express both 85 kDa cytosolic and 14 kDa secretory phospholipase A2++. The 85 kDa phospholipase A2 was induced by interleukin-1beta, whereas there was no apparent change in secretory phospholipase A2 enzyme concentrations. Both the secretory phospholipase A2 inhibitor p-bromophenylacyl bromide and the cytosolic phospholipase A2 inhibitor arachidonyl trifluoromethyl ketone decreased basal and interleukin-1beta-stimulated arachidonic acid release. In contrast, only inhibition of cytosolic phospholipase A2 led to a decrease in interleukin-1beta-stimulated prostaglandin E2 release. Basal and interleukin-1beta-stimulated arachidonic acid release appears to be the result of the activity of both cytosolic and secretory phospholipase A2. Interleukin-1beta-stimulated prostaglandin E2 release appears to be dependent on the activity of cytosolic phospholipase A2.
Authors:
E M Grossmann; W E Longo; J E Mazuski; N Panesar; D L Kaminski
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Journal of gastrointestinal surgery : official journal of the Society for Surgery of the Alimentary Tract     Volume:  4     ISSN:  1091-255X     ISO Abbreviation:  J. Gastrointest. Surg.     Publication Date:    2000 Mar-Apr
Date Detail:
Created Date:  2000-06-30     Completed Date:  2000-06-30     Revised Date:  2007-11-15    
Medline Journal Info:
Nlm Unique ID:  9706084     Medline TA:  J Gastrointest Surg     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  193-200     Citation Subset:  IM    
Affiliation:
Theodore Cooper Surgical Research Institute, Saint Louis University School of Medicine, St. Louis, MO 63110-0250, USA.
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MeSH Terms
Descriptor/Qualifier:
Arachidonic Acid / metabolism*
Blotting, Western
Cytosol / enzymology
Dinoprostone / metabolism*
Epithelial Cells / drug effects*,  metabolism
Gallbladder / cytology,  enzymology*,  metabolism
Gallbladder Neoplasms / enzymology,  metabolism
Humans
Interleukin-1 / pharmacology
Phospholipases A / antagonists & inhibitors,  metabolism*,  secretion
Phospholipases A2
Tumor Cells, Cultured / drug effects
Grant Support
ID/Acronym/Agency:
DK 27695/DK/NIDDK NIH HHS
Chemical
Reg. No./Substance:
0/Interleukin-1; 363-24-6/Dinoprostone; 506-32-1/Arachidonic Acid; EC 3.1.1.-/Phospholipases A; EC 3.1.1.4/Phospholipases A2

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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