| Role of cytochrome P-450 and flavin-containing monooxygenase in the N-hydroxylation of N-methyl-4-aminoazobenzene in rat liver: analysis with purified enzymes and antibodies. | |
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MedLine Citation:
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PMID: 6510637 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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By means of high pressure liquid chromatography, the role of flavin-containing monooxygenase (FMO) and cytochrome P-450 (cyt. P-450) in the metabolism of N-methyl-4-aminoazobenzene (MAB) by rat liver microsomes in vitro was studied with the help of antibodies and a chemical inhibitor. Antibody against cyt. P-488 from 3-methylcholanthrene-treated rats (MC-P-448) decreased the formation of N-hydroxy-N-methyl-4-aminoazobenzene (N-OH-MAB) by about 30% in microsomes from MC-treated rats (MC-microsomes), but showed no inhibitory effect on the formation of N-OH-MAB in microsomes from untreated rats (untreated microsomes) or in microsomes from phenobarbital-treated rats (PB-microsomes). Antibody against cyt. P-450 from PB-treated rats did not inhibit N-hydroxylation of MAB by any of the microsomes tested. A competitive inhibitor of FMO, methimazole, inhibited the N-hydroxylation of MAB by 65% in the case of MC-microsomes, and the residual activity was inhibited completely by anti-NADPH-cytochrome P-450 reductase (anti-fPT) antibody. These results indicate that in MC-microsomes, the N-hydroxylation of MAB is catalyzed by both FMO and MC-P-448, but in untreated and PB-microsomes the reaction is catalyzed exclusively by FMO. |
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Authors:
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T Kimura; M Kodama; C Nagata |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Gann = Gan Volume: 75 ISSN: 0016-450X ISO Abbreviation: Gann Publication Date: 1984 Oct |
Date Detail:
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Created Date: 1985-02-04 Completed Date: 1985-02-04 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 8214471 Medline TA: Gann Country: JAPAN |
Other Details:
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Languages: eng Pagination: 895-904 Citation Subset: IM |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Antigen-Antibody Complex Aryl Hydrocarbon Hydroxylases / metabolism* Azo Compounds / metabolism* Biotransformation Carbon Radioisotopes Carcinogens / metabolism* Cytochrome P-450 Enzyme System / metabolism* Hydroxylation Immune Sera Kinetics Male Methylcholanthrene / pharmacology Microsomes, Liver / drug effects, enzymology* Rats Rats, Inbred Strains p-Aminoazobenzene / analogs & derivatives, metabolism* |
| Chemical | |
Reg. No./Substance:
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0/Antigen-Antibody Complex; 0/Azo Compounds; 0/Carbon Radioisotopes; 0/Carcinogens; 0/Immune Sera; 56-49-5/Methylcholanthrene; 60-09-3/p-Aminoazobenzene; 621-90-9/N-methyl-4-aminoazobenzene; 9035-51-2/Cytochrome P-450 Enzyme System; EC 1.14.14.1/Aryl Hydrocarbon Hydroxylases |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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