| Role for cysteinyl leukotrienes in allergen-induced change in circulating dendritic cell number in asthma. | |
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MedLine Citation:
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PMID: 15241347 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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BACKGROUND: Dendritic cells are important antigen-presenting cells. After an allergen inhalation, their numbers rapidly decrease in circulation and increase in the airway mucosa. OBJECTIVE: To investigate whether allergen-induced changes in the number of circulating dendritic cells are mediated by cysteinyl leukotrienes. METHODS: In a randomized, double-blind, crossover study, we examined the effects of 2 weeks of treatment with pranlukast (a cysteinyl leukotriene 1 [CysLT1] receptor antagonist) 300 mg twice daily and placebo on allergen-induced changes in airway responses and circulating dendritic cells in 15 subjects with mild asthma. We examined by flow cytometry, before and at 3 hours and 24 hours after allergen inhalation, the proportion of myeloid (CD33+) and plasmacytoid (CD123+) dendritic cells (HLA-DR+, CD14-, CD16-) among all peripheral blood mononuclear cells. The fraction of dendritic cells expressing CysLT1 receptor was also determined. RESULTS: Compared with placebo, pranlukast significantly attenuated both the maximum early (by 55%) and the late (by 39%) asthma responses, the allergen-induced methacholine airway hyperresponsiveness, and the increase in macrophage inflammatory protein 1alpha and 3alpha in induced sputum. A significantly greater proportion of CD33+ cells (55%) expressed CysLT1 receptor compared with CD123+ cells (11%). Consistent with this, pranlukast prevented the allergen-induced decrease in CD33+ dendritic cells at 3 hours postallergen (mean Delta from baseline, +4.4%) compared with placebo (mean Delta, -8.4; P <.05), but not CD123+ cells. CONCLUSION: Pretreatment with pranlukast attenuated allergen-induced airway responses and the decrease in circulating myeloid dendritic cells, demonstrating a novel role of cysteinyl leukotrienes in dendritic cell trafficking. |
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Authors:
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Krishnan Parameswaran; Hong Liang; Adrian Fanat; Richard Watson; Denis P Snider; Paul M O'Byrne |
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Publication Detail:
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Type: Clinical Trial; Journal Article; Randomized Controlled Trial; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: The Journal of allergy and clinical immunology Volume: 114 ISSN: 0091-6749 ISO Abbreviation: J. Allergy Clin. Immunol. Publication Date: 2004 Jul |
Date Detail:
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Created Date: 2004-07-08 Completed Date: 2004-09-28 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 1275002 Medline TA: J Allergy Clin Immunol Country: United States |
Other Details:
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Languages: eng Pagination: 73-9 Citation Subset: AIM; IM |
Copyright Information:
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Copyright 2004 American Academy of Allergy, Asthma and Immunology |
Affiliation:
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Firestone Institute for Respiratory Health, St. Joseph's Healthcare, Department of Medicine, McMaster University, Hamilton, Canada. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Adult Allergens / adverse effects, immunology Asthma / drug therapy, immunology* Bronchial Hyperreactivity / drug therapy, immunology Cell Count Chemotactic Factors / immunology Chromones / pharmacology*, therapeutic use Cross-Over Studies Dendritic Cells / drug effects*, immunology Double-Blind Method Humans Leukotriene Antagonists / pharmacology*, therapeutic use Membrane Proteins / drug effects, immunology* Middle Aged Receptors, Leukotriene / drug effects, immunology* Sputum / chemistry, immunology |
| Chemical | |
Reg. No./Substance:
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0/Allergens; 0/Chemotactic Factors; 0/Chromones; 0/Leukotriene Antagonists; 0/Membrane Proteins; 0/Receptors, Leukotriene; 0/leukotriene D4 receptor; 0/pranlukast |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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