Document Detail

Role of cyclin inhibitor protein p21 in the inhibition of HCT116 human colon cancer cell proliferation by American ginseng (Panax quinquefolius) and its constituents.
MedLine Citation:
PMID:  19674880     Owner:  NLM     Status:  MEDLINE    
American ginseng and its ginsenoside constituents have been shown to exert anti-cancer effects although the mechanism of action remains unclear. The present study determined the effects of water-extracted ginseng (GE) or its ginsenoside (GF) and polysaccharide (PS) fractions on the proliferation of human colon cancer cells and examined the role of p21 in mediating these effects using wild-type and p21-/- HCT116 human colon carcinoma cells. Proliferation was inhibited by GE, GF, and PS in wild-type and p21-/- cells, and the p21-/- cells were more sensitive to these treatments. Wild type cells treated with GE were arrested in the G0/G1 phase of the cell cycle and the expression of p53 and p21 proteins was increased while phospho-MEK levels decreased. In contrast, cells deficient in p21 displayed reduced cell viability, elevated number of dead cells, and increased expression of Bax and cleaved caspase-3 proteins. Both polysaccharides and ginsenosides appear to be responsible for the anti-proliferative and proapoptotic effects of GE. This study suggests that p21 functions to arrest HCT116 wild-type cells treated with GE, while p21-deficient cells undergo cell death in a ginseng constituent-dependent manner.
M L King; L L Murphy
Publication Detail:
Type:  Journal Article     Date:  2009-08-11
Journal Detail:
Title:  Phytomedicine : international journal of phytotherapy and phytopharmacology     Volume:  17     ISSN:  1618-095X     ISO Abbreviation:  Phytomedicine     Publication Date:  2010 Mar 
Date Detail:
Created Date:  2010-02-15     Completed Date:  2010-08-30     Revised Date:  2014-09-21    
Medline Journal Info:
Nlm Unique ID:  9438794     Medline TA:  Phytomedicine     Country:  Germany    
Other Details:
Languages:  eng     Pagination:  261-8     Citation Subset:  IM    
Copyright Information:
Copyright 2009 Elsevier GmbH. All rights reserved.
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MeSH Terms
Antineoplastic Agents, Phytogenic / pharmacology,  therapeutic use*
Apoptosis / drug effects
Caspase 3 / metabolism
Cell Cycle / drug effects
Cell Proliferation / drug effects*
Cell Survival / drug effects
Colonic Neoplasms / drug therapy*,  metabolism
Cyclin-Dependent Kinase Inhibitor p21 / genetics,  metabolism*
Ginsenosides / pharmacology,  therapeutic use*
HCT116 Cells
Panax / chemistry*
Phosphotransferases / metabolism
Plant Extracts / chemistry,  pharmacology,  therapeutic use
Plant Roots
Polysaccharides / pharmacology,  therapeutic use*
Tumor Suppressor Protein p53 / metabolism
bcl-2-Associated X Protein / metabolism
Grant Support
R21 CA121074/CA/NCI NIH HHS; R21 CA121074-01/CA/NCI NIH HHS
Reg. No./Substance:
0/Antineoplastic Agents, Phytogenic; 0/Cyclin-Dependent Kinase Inhibitor p21; 0/Ginsenosides; 0/Plant Extracts; 0/Polysaccharides; 0/Tumor Suppressor Protein p53; 0/bcl-2-Associated X Protein; EC 2.7.-/Phosphotransferases; EC 3.4.22.-/Caspase 3

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