| Role of constitutive nitric oxide synthase S-nitrosylation in Helicobacter pylori-induced gastric mucosal cell apoptosis: effect of ghrelin. | |
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MedLine Citation:
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PMID: 20596895 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Infection with H. pylori is a primary factor in the etiology of gastric disease, and the excessive NO generation and a massive rise in apoptosis are well recognized features that characterize the mucosal inflammatory responses to the bacterium and its lipopolysaccharide (LPS). Here, we report that H. pylori LPS-induced enhancement in gastric mucosal cell apoptosis and NO generation was associated with the suppression in constitutive nitric oxide synthase (cNOS) activity and a marked up-regulation in the activity of inducible nitric oxide synthase (iNOS). Further, we demonstrate that the detrimental effect of the LPS on cNOS was manifested in the enzyme protein S-nitrosylation, that was susceptible to suppression by iNOS inhibitor, 1400W. Moreover, we show that the countering effect of peptide hormone, ghrelin, on the LPS-induced changes in apoptosis and cNOS activity was reflected in the loss in cNOS S-nitrosylation and the increase in the enzyme phosphorylation. These findings demonstrate that the disturbances in gastric mucosal NO generation system caused by H. pylori result from the iNOS-derived NO suppression of cNOS activation through S-nitrosylation. We also report that ghrelin protection against H. pylori-induced gastric mucosal proapoptotic events involves cNOS activation manifested by the increase in enzyme protein phosphorylation and a decrease in its S-nitrosylation. |
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Authors:
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B L Slomiany; A Slomiany |
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Publication Detail:
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Type: Journal Article Date: 2010-07-02 |
Journal Detail:
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Title: Inflammopharmacology Volume: 18 ISSN: 1568-5608 ISO Abbreviation: Inflammopharmacology Publication Date: 2010 Oct |
Date Detail:
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Created Date: 2010-09-20 Completed Date: 2010-12-28 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 9112626 Medline TA: Inflammopharmacology Country: Switzerland |
Other Details:
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Languages: eng Pagination: 233-40 Citation Subset: IM |
Affiliation:
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Research Center, New Jersey Dental School, University of Medicine and Dentistry of New Jersey, Newark, NJ 07103-2400, USA. slomiabr@umdnj.edu |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Amidines
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pharmacology Animals Apoptosis / drug effects* Benzylamines / pharmacology Cells, Cultured Gastric Mucosa / cytology*, drug effects, metabolism Ghrelin / metabolism*, pharmacology Helicobacter pylori / physiology* Lipopolysaccharides / pharmacology Nitric Oxide / metabolism Nitric Oxide Synthase / metabolism* Nitric Oxide Synthase Type II / antagonists & inhibitors, metabolism Phosphorylation Rats |
| Chemical | |
Reg. No./Substance:
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0/Amidines; 0/Benzylamines; 0/Ghrelin; 0/Lipopolysaccharides; 0/N-(3-(aminomethyl)benzyl)acetamidine; 10102-43-9/Nitric Oxide; EC 1.14.13.39/Nitric Oxide Synthase; EC 1.14.13.39/Nitric Oxide Synthase Type II |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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