| Role of colanic acid exopolysaccharide in the survival of enterohaemorrhagic Escherichia coli O157:H7 in simulated gastrointestinal fluids. | |
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MedLine Citation:
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PMID: 16706906 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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AIM: This study evaluated the production of colanic acid (CA) exopolysaccharide (EPS) by Escherichia coli O157:H7 in relation to the pathogen's ability to survive under acidic conditions simulating the environment in the human gastrointestinal tract. METHODS AND RESULTS: Escherichia coli O157:H7 W6-13 and its CA-deficient mutant M4020 were examined for their resistance to bile salts, and their ability to survive in simulated gastric fluid containing pepsin (pH 2.0) and simulated intestinal fluid containing pancreatin (pH 8.0). The effect of acid adaptation at pH 5.5 on the survival of E. coli O157:H7 in simulated gastric fluid was also determined. The results indicated that the survivability of M4020, under conditions simulating the environment in the human gastrointestinal tract, reduced more drastically than the viability of W6-13. The presence of bile salts had a slight effect on both types of E. coli O157:H7 cells. The loss of CA did not change the ability of M4020 to respond to acid adaptation. CONCLUSION: The EPS CA may serve as a protective barrier to E. coli O157:H7 for its survival in the human gastrointestinal tract. SIGNIFICANCE AND IMPACT OF THE STUDY: The study contributes to a better understanding of the EPS affecting the ability of E. coli O157:H7 to combat acid stress. |
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Authors:
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Y Mao; M P Doyle; J Chen |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S. |
Journal Detail:
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Title: Letters in applied microbiology Volume: 42 ISSN: 0266-8254 ISO Abbreviation: Lett. Appl. Microbiol. Publication Date: 2006 Jun |
Date Detail:
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Created Date: 2006-05-18 Completed Date: 2006-07-19 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 8510094 Medline TA: Lett Appl Microbiol Country: England |
Other Details:
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Languages: eng Pagination: 642-7 Citation Subset: IM |
Affiliation:
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Center for Food Safety and Department of Food Science and Technology, The University of Georgia, Griffin, GA 30223-1797, USA. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Escherichia coli O157
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drug effects,
growth & development* Gastric Juice / microbiology*, secretion Heat-Shock Response Humans Hydrogen-Ion Concentration Intestinal Secretions / microbiology* Polysaccharides / metabolism*, pharmacology Polysaccharides, Bacterial / metabolism |
| Chemical | |
Reg. No./Substance:
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0/Polysaccharides; 0/Polysaccharides, Bacterial; 9012-87-7/colanic acid |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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