Document Detail


Role of class I and class II histone deacetylases in carcinoma cells using siRNA.
MedLine Citation:
PMID:  14521942     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The role of the individual histone deacetylases (HDACs) in the regulation of cancer cell proliferation was investigated using siRNA-mediated protein knockdown. The siRNA for HDAC3 and HDAC1 demonstrated significant morphological changes in HeLa S3 consistent with those observed with HDAC inhibitors. SiRNA for HDAC 4 or 7 produced no morphological changes in HeLa S3 cells. HDAC1 and 3 siRNA produced a concentration-dependent inhibition of HeLa cell proliferation; whereas, HDAC4 and 7 siRNA showed no effect. HDAC3 siRNA caused histone hyperacetylation and increased the percent of apoptotic cells. These results demonstrate that the Class I HDACs such as HDACs 1 and 3 are important in the regulation of proliferation and survival in cancer cells. These results and the positive preclinical results with non-specific inhibitors of the HDAC enzymes provide further support for the development of Class I selective HDAC inhibitors as cancer therapeutics.
Authors:
Keith B Glaser; Junling Li; Michael J Staver; Ru-Qi Wei; Daniel H Albert; Steven K Davidsen
Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Biochemical and biophysical research communications     Volume:  310     ISSN:  0006-291X     ISO Abbreviation:  Biochem. Biophys. Res. Commun.     Publication Date:  2003 Oct 
Date Detail:
Created Date:  2003-10-02     Completed Date:  2003-11-25     Revised Date:  2009-11-19    
Medline Journal Info:
Nlm Unique ID:  0372516     Medline TA:  Biochem Biophys Res Commun     Country:  United States    
Other Details:
Languages:  eng     Pagination:  529-36     Citation Subset:  IM    
Affiliation:
Cancer Research, R47J-AP9, Global Pharmaceutical Research and Development, Abbott Laboratories, Abbott Park, IL 60064-6121, USA. keith.glaser@abbott.com
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MeSH Terms
Descriptor/Qualifier:
Acetylation
Antineoplastic Agents / pharmacology
Apoptosis
Carcinoma / enzymology*,  genetics,  pathology
Cell Division / drug effects
Hela Cells
Histone Deacetylases / classification,  genetics,  physiology*
Histones / metabolism
Humans
RNA Interference
RNA, Small Interfering / pharmacology
Chemical
Reg. No./Substance:
0/Antineoplastic Agents; 0/Histones; 0/RNA, Small Interfering; EC 3.5.1.98/Histone Deacetylases

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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