Document Detail


Role of class B scavenger receptor type I in phagocytosis of apoptotic rat spermatogenic cells by Sertoli cells.
MedLine Citation:
PMID:  10026214     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Rat Sertoli cells phagocytose apoptotic spermatogenic cells, which consist mostly of spermatocytes, in primary culture by recognizing phosphatidylserine (PS) exposed on the surface of degenerating spermatogenic cells. We compared the mode of phagocytosis using spermatogenic cells at different stages of spermatogenesis. Spermatogenic cells were separated into several groups based on their ploidy, with purities of 60-90%. When the fractionated spermatogenic cell populations were subjected to a phagocytosis assay, cells with ploidies of 1n, 2n, and 4n were almost equally phagocytosed by Sertoli cells. All the cell populations exposed PS on the cell surface, and phagocytosis of all cell populations was similarly inhibited by the addition of PS-containing liposomes. Class B scavenger receptor type I (SR-BI), a candidate for the PS receptor, was detected in Sertoli cells. Overexpression of the rat SR-BI cDNA increased the PS-mediated phagocytic activity of Sertoli cell-derived cell lines. Moreover, phagocytosis of spermatogenic cells by Sertoli cells was inhibited in the presence of an anti-SR-BI antibody. Finally, the addition of high density lipoprotein, a ligand specific for SR-BI, decreased both phagocytosis of spermatogenic cells and incorporation of PS-containing liposomes by Sertoli cells. In conclusion, SR-BI functions at least partly as a PS receptor, enabling Sertoli cells to recognize and phagocytose apoptotic spermatogenic cells at all stages of differentiation.
Authors:
A Shiratsuchi; Y Kawasaki; M Ikemoto; H Arai; Y Nakanishi
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  The Journal of biological chemistry     Volume:  274     ISSN:  0021-9258     ISO Abbreviation:  J. Biol. Chem.     Publication Date:  1999 Feb 
Date Detail:
Created Date:  1999-03-18     Completed Date:  1999-03-18     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  2985121R     Medline TA:  J Biol Chem     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  5901-8     Citation Subset:  IM    
Affiliation:
Faculty of Pharmaceutical Sciences, Kanazawa University, Kanazawa, Ishikawa 920-0934, Japan.
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MeSH Terms
Descriptor/Qualifier:
Animals
Antigens, CD36 / genetics,  metabolism,  physiology*
Apoptosis*
Cloning, Molecular
DNA, Complementary
Male
Membrane Proteins*
Phagocytosis / physiology*
Phosphatidylserines / metabolism
Ploidies
Rats
Receptors, Cell Surface / metabolism
Receptors, Immunologic*
Receptors, Lipoprotein*
Receptors, Scavenger
Scavenger Receptors, Class B
Sertoli Cells / metabolism*,  pathology
Spermatocytes / pathology*
Chemical
Reg. No./Substance:
0/Antigens, CD36; 0/DNA, Complementary; 0/Membrane Proteins; 0/Phosphatidylserines; 0/Receptors, Cell Surface; 0/Receptors, Immunologic; 0/Receptors, Lipoprotein; 0/Receptors, Scavenger; 0/Scarb1 protein, mouse; 0/Scarb1 protein, rat; 0/Scavenger Receptors, Class B

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