Document Detail

Role of chromatin phospholipids on template availability and ultrastructure of isolated nuclei.
MedLine Citation:
PMID:  6180606     Owner:  NLM     Status:  MEDLINE    
The influence of phospholipid vesicles has been tested on isolated nuclei by evaluating the endogenous DNA-dependent RNA polymerase and the chromatin ultrastructure. Negatively charged phosphatidylserine liposomes have a stimulating effect on RNA synthesis, while the vesicles obtained with the neutral sphingomyelin, phosphatidylethanolamine and phosphatidylcholine, and the acidic phosphatidylinositol, are inhibitory. The enhancement of transcription by phosphatidylserine seems to be due to both elongation and initiation of RNA transcripts and very likely depends on interactions of the lipid with the template rather than with the enzymes. The morphological analysis indicates deep changes of the chromatin organization, mainly concerning the size and distribution of the fibers, without any variation of the nuclear volume. The rearrangement of the chromatin could account for the variations of the template availability for RNA synthesis induced by the vesicles and might be associated with changes of the nuclear matrix.
F A Manzoli; S Capitani; G Mazzotti; O Barnabei; N M Maraldi
Related Documents :
3401376 - Bovine thymus satellite i dna sequences, which are highly methylated, are preferentiall...
20012576 - Chromatin assembly and signalling the end of dna repair requires acetylation of histone...
3305026 - Endogenous polyamines are intimately associated with highly condensed chromatin in vivo...
16220346 - Replication of heterochromatin: insights into mechanisms of epigenetic inheritance.
8816496 - Dna length is a critical parameter for eukaryotic transcription in vivo.
19066286 - Spatial organization of nucleotide excision repair proteins after uv-induced dna damage...
Publication Detail:
Type:  In Vitro; Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Advances in enzyme regulation     Volume:  20     ISSN:  0065-2571     ISO Abbreviation:  Adv. Enzyme Regul.     Publication Date:  1982  
Date Detail:
Created Date:  1982-10-29     Completed Date:  1982-10-29     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0044263     Medline TA:  Adv Enzyme Regul     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  247-62     Citation Subset:  IM    
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Cell Nucleus / ultrastructure*
Chromatin / physiology*
DNA-Directed RNA Polymerases / metabolism
Ions / pharmacology
Liver / ultrastructure
Phospholipids / physiology*
RNA / biosynthesis
Templates, Genetic
Reg. No./Substance:
0/Chromatin; 0/Ions; 0/Liposomes; 0/Phospholipids; 63231-63-0/RNA; EC RNA Polymerases

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Previous Document:  Regulation of nucleotide and pentose synthesis in resting and stimulated 3T6 fibroblasts.
Next Document:  Elevation of quinone reductase activity by anticarcinogenic antioxidants.