Document Detail

Role of the charged tail in localization of a surface protein antigen of Streptococcus mutans.
MedLine Citation:
PMID:  9119499     Owner:  NLM     Status:  MEDLINE    
To make clear the role of the C terminus of a surface protein antigen (PAc) of Streptococcus mutans, stepwise truncations beginning at the C terminus of PAc were performed by utilizing site-directed mutagenesis. A remarkable increase in the amount of cell-free PAc was observed upon deletion of four or more amino acid residues at the C terminus. On the other hand, the amount of cell surface PAc gradually decreased when increasing numbers (four or more) of amino acid residues were deleted at the C terminus, and deletion of six amino acids involving both the total charged tail and Leu, an amino acid residue immediately upstream of the charged tail, resulted in a drastic reduction in the amount of cell surface PAc. These results indicate that the cytoplasmic charged tail and Leu residue are required for cell surface localization of PAc in S. mutans.
Y Murakami; Y Yamashita; Y Nakano; H O Ito; H Yu; T Koga
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Infection and immunity     Volume:  65     ISSN:  0019-9567     ISO Abbreviation:  Infect. Immun.     Publication Date:  1997 Apr 
Date Detail:
Created Date:  1997-04-24     Completed Date:  1997-04-24     Revised Date:  2009-11-18    
Medline Journal Info:
Nlm Unique ID:  0246127     Medline TA:  Infect Immun     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  1531-5     Citation Subset:  IM    
Department of Preventive Dentistry, Kyushu University Faculty of Dentistry, Higashi-ku, Fukuoka, Japan.
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MeSH Terms
Antigens, Bacterial / genetics*
Bacterial Outer Membrane Proteins / genetics*
Bacterial Proteins*
Blotting, Western
Membrane Glycoproteins*
Mutagenesis, Site-Directed
Streptococcus / genetics*,  immunology
Reg. No./Substance:
0/Antigens, Bacterial; 0/Bacterial Outer Membrane Proteins; 0/Bacterial Proteins; 0/Membrane Glycoproteins; 0/S-layer proteins

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