| Role of cellular acidosis in production of nitric oxide in canine ischemic myocardium. | |
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MedLine Citation:
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PMID: 11549351 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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We tested the hypothesis that cellular acidosis modulates the production of nitric oxide (NO) in ischemic hearts. In canine hearts, we decreased coronary blood flow (CBF) to one third of the control by reduction of coronary perfusion pressure (105+/-3 to 41+/-5 mmHg), and thereafter we maintained CBF constant (89.8+/-1.6 to 30.0+/-0.5 ml/100 g/min) with an intracoronary administration of either saline, atropine, rauwolscine, HOE140, 8-sulfophenyltheophylline (8SPT), NaHCO3, or HOE642 (the inhibitor of Na+/H+ exchange). The cardiac NO levels defined as the differences of the nitrate and nitrite levels between coronary venous and arterial blood increased in the saline administration (2.9+/-0.2 to 12.7+/-1.7 micromol/l), and the extents of increases were identical in the condition of either saline, atropine, rauwolscine, HOE140 or 8SPT administration. In the condition with either NaHCO3 or HOE642, the increases in the cardiac NO levels were blunted (4.5+/-0.7 and 4.8+/-0.4 micromol/l, respectively). Cyclic GMP content of epicardial coronary artery in the ischemic area increased, which was also attenuated by either NaHCO3 or HOE642. We confirmed the acidosis-induced NO production in a more severe ischemic myocardium, and also showed that cellular acidosis produced by infusion of HCl increased NO production in non-ischemic myocardium. We conclude that cellular acidosis and subsequent activation of Na+/H+ exchanges modulate production of endogenous NO in canine ischemic myocardium. |
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Authors:
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M Kitakaze; K Node; S Takashima; H Asanuma; M Asakura; S Sanada; Y Shinozaki; H Mori; H Sato; T Kuzuya; M Hori |
Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Journal of molecular and cellular cardiology Volume: 33 ISSN: 0022-2828 ISO Abbreviation: J. Mol. Cell. Cardiol. Publication Date: 2001 Sep |
Date Detail:
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Created Date: 2001-09-10 Completed Date: 2002-01-03 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 0262322 Medline TA: J Mol Cell Cardiol Country: England |
Other Details:
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Languages: eng Pagination: 1727-37 Citation Subset: IM |
Copyright Information:
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Copyright 2001 Academic Press. |
Affiliation:
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Department of Internal Medicine and Therapeutics, Osaka University Graduate School of Medicine, Suita, Japan. kitakaze@medone.osaka-u.ac.jp |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Acidosis
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metabolism* Animals Anti-Arrhythmia Agents / pharmacology Bicarbonates / pharmacology Coronary Circulation / physiology* Coronary Vessels / drug effects, metabolism Cyclic GMP / metabolism Dogs Enzyme Inhibitors / pharmacology Guanidines / pharmacology Heart / drug effects, physiopathology Hydrochloric Acid / pharmacology Myocardial Ischemia / metabolism* Myocardium / metabolism* NG-Nitroarginine Methyl Ester / pharmacology Nitric Oxide / biosynthesis* Sulfones / pharmacology |
| Chemical | |
Reg. No./Substance:
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0/Anti-Arrhythmia Agents; 0/Bicarbonates; 0/Enzyme Inhibitors; 0/Guanidines; 0/Sulfones; 0/cariporide; 10102-43-9/Nitric Oxide; 50903-99-6/NG-Nitroarginine Methyl Ester; 7647-01-0/Hydrochloric Acid; 7665-99-8/Cyclic GMP |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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