| Role of calmodulin and myosin light chain kinase in the activation of carbachol-activated cationic current in murine ileal myocytes. | |
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MedLine Citation:
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PMID: 18066127 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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We investigated the effect of calmodulin (CaM) and myosin light chain kinase (MLCK) on murine ileal myocytes using the whole-cell patch-clamp technique. Under the voltage clamp, at the holding potential of -60 mV, 50 micromol/L carbachol (CCh) induced inward currents (I CCh), and spontaneous decay of I CCh occurred. The peak inward currents induced by the repetitive application of CCh (50 micromol/L) tended to decrease in amplitude. Intracellular application of 0.2 mmol/L guanosine 5'-O-(gamma-thio)triphosphate (GTP gammaS) from the patch electrode induced an inward current at a holding potential of -60m V, and the peak inward currents induced by the repetitive application of Cs tended to decrease slightly in amplitude. The amplitude of I CCh was reduced by pretreatment either with W-7, trifluoroperazine, W-5, and melittin (CaM inhibitors) or with ML-7 and ML-9 (selective MLCK inhibitors), and the inhibitory effects were reversible. However, when we pretreated with 50 micromol/L W-7 or 5 micromol/L ML-7 on GTP gammaS-induced inward currents, almost no inhibition was observed in the inward currents. Application of both Rho kinase inhibitor and MLCK inhibitor inhibited GTP gammaS-induced currents. We conclude that CaM and MLCK modulate the activation process of I CCh in murine ileal myocytes and suggest that the classical type transient receptor potential (TRPC) channel 5 might be a candidate for nonselective cationic currents (NSCC) activated by muscarinic stimulation in gastrointestinal smooth muscle cells. |
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Authors:
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Byung Joo Kim; Ju-Hong Jeon; Seon Jeong Kim; Insuk So |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Canadian journal of physiology and pharmacology Volume: 85 ISSN: 0008-4212 ISO Abbreviation: Can. J. Physiol. Pharmacol. Publication Date: 2007 Dec |
Date Detail:
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Created Date: 2007-12-10 Completed Date: 2008-04-01 Revised Date: 2009-11-19 |
Medline Journal Info:
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Nlm Unique ID: 0372712 Medline TA: Can J Physiol Pharmacol Country: Canada |
Other Details:
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Languages: eng Pagination: 1254-62 Citation Subset: IM |
Affiliation:
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Center for Bio-Artificial Muscle and Department of Physiology, Seoul National University College of Medicine, Seoul 110-799, Korea. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Azepines / pharmacology Calmodulin / antagonists & inhibitors, physiology* Carbachol / pharmacology Cells, Cultured Female Guanosine 5'-O-(3-Thiotriphosphate) / pharmacology Ileum / cytology, physiology* Male Mice Mice, Inbred ICR Myocytes, Smooth Muscle / drug effects, physiology* Myosin-Light-Chain Kinase / antagonists & inhibitors, physiology* Naphthalenes / pharmacology Sulfonamides / pharmacology TRPC Cation Channels / physiology |
| Chemical | |
Reg. No./Substance:
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0/Azepines; 0/Calmodulin; 0/Naphthalenes; 0/Sulfonamides; 0/TRPC Cation Channels; 0/Trpc5 protein, mouse; 109376-83-2/ML 7; 37589-80-3/Guanosine 5'-O-(3-Thiotriphosphate); 51-83-2/Carbachol; 65595-90-6/W 7; EC 2.7.11.18/Myosin-Light-Chain Kinase |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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