| Role of calcium-independent phospholipases (iPLA(2)) in phosphatidylcholine metabolism. | |
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MedLine Citation:
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PMID: 11563837 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The proposed role of calcium-independent phospholipase A(2) (iPLA(2)) in membrane phospholipid homeostasis was tested by examining the perturbation of phosphatidylcholine metabolism by enzyme overexpression. There are alternatively spliced forms of murine iPLA(2) that were widely expressed in mouse tissues: a long form containing exon-9 that is membrane-associated and a short form lacking exon-9 that is distributed between the membrane and cytosolic fractions. Enforced expression of either iPLA(2) isoform led to a significant increase in intracellular free fatty acid, lysophosphatidylcholine, and GPC without a concomitant increase in the incorporation of either exogenous arachidonic acid or choline. The accumulation of lysophosphatidylcholine in iPLA(2)-expressing cells illustrates the limited capacity of cells for reacylation and degradation of lysophospholipids. Since iPLA(2) overexpression did not accelerate either phospholipid remodeling or phosphatidylcholine synthesis, this enzyme does play a determinant (rate-controlling?) role in either of these cellular processes. |
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Authors:
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C H Chiu; S Jackowski |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
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Title: Biochemical and biophysical research communications Volume: 287 ISSN: 0006-291X ISO Abbreviation: Biochem. Biophys. Res. Commun. Publication Date: 2001 Sep |
Date Detail:
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Created Date: 2001-09-20 Completed Date: 2001-11-01 Revised Date: 2007-11-15 |
Medline Journal Info:
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Nlm Unique ID: 0372516 Medline TA: Biochem Biophys Res Commun Country: United States |
Other Details:
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Languages: eng Pagination: 600-6 Citation Subset: IM |
Copyright Information:
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Copyright 2001 Academic Press. |
Affiliation:
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Protein Science Division, St. Jude Children's Research Hospital, Memphis, Tennessee 38105-2794, USA. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Alternative Splicing Animals COS Cells Exons Fatty Acids / metabolism Group VI Phospholipases A2 Immunoblotting Lysophosphatidylcholines / metabolism Mice Phosphatidylcholines / metabolism* Phospholipases A / metabolism, physiology* Plasmids / metabolism Protein Isoforms Reverse Transcriptase Polymerase Chain Reaction Subcellular Fractions Tissue Distribution Transfection |
| Grant Support | |
ID/Acronym/Agency:
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CA21765/CA/NCI NIH HHS; GM45737/GM/NIGMS NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Fatty Acids; 0/Lysophosphatidylcholines; 0/Phosphatidylcholines; 0/Protein Isoforms; EC 3.1.1.-/Phospholipases A; EC 3.1.1.4/Group VI Phospholipases A2; EC 3.1.1.4/Pla2g6 protein, mouse |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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