| Role of beta-D-galactofuranose in Leishmania major macrophage invasion. | |
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MedLine Citation:
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PMID: 12438330 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The role of glycosylinositol phospholipid 1 (GIPL-1) of Leishmania (Leishmania) major in the interaction of promastigotes and amastigotes with macrophages was analyzed. Monoclonal antibody MEST-1, which recognizes glycolipids containing terminal galactofuranose (Galf) residues (E. Suzuki, M. S. Toledo, H. K. Takahashi, and A. H. Straus, Glycobiology 7:463-468, 1997), was used to detect GIPL-1 in Leishmania by indirect immunofluorescence and to analyze its role in macrophage infectivity. L. major promastigotes showed intense fluorescence with MEST-1, and GIPL-1 was detected in both amastigote and promastigote forms by high-performance thin-layer chromatography immunostaining by using MEST-1. Delipidation of L. major promastigotes with isopropanol-hexane-water eliminated the MEST-1 reactivity, confirming that only GIPL-1 is recognized in either amastigotes or promastigotes of this species. The biological role of GIPL-1 in the ability of L. major to invade macrophages was studied by using either Fab fragments of MEST-1 or methylglycosides. Preincubation of parasites with Fab fragments reduced macrophage infectivity in about 80% of the promastigotes and 30% of the amastigotes. Preincubation of peritoneal macrophages with p-nitrophenyl-beta-galactofuranoside (10 mM) led to significant ( approximately 80%) inhibition of promastigote infectivity. These data suggest that a putative new receptor recognizing beta-D-Galf is associated with L. major macrophage infectivity and that GIPL-1 containing a terminal Galf residue is involved in the L. major-macrophage interaction. |
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Authors:
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Erika Suzuki; Ameria K Tanaka; Marcos S Toledo; Helio K Takahashi; Anita H Straus |
Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Infection and immunity Volume: 70 ISSN: 0019-9567 ISO Abbreviation: Infect. Immun. Publication Date: 2002 Dec |
Date Detail:
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Created Date: 2002-11-19 Completed Date: 2003-01-07 Revised Date: 2013-04-18 |
Medline Journal Info:
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Nlm Unique ID: 0246127 Medline TA: Infect Immun Country: United States |
Other Details:
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Languages: eng Pagination: 6592-6 Citation Subset: IM |
Affiliation:
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Department of Biochemistry, Universidade Federal de São Paulo/Escola Paulista de Medicina, Brazil. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Antibodies, Monoclonal / immunology* Antibodies, Protozoan / immunology* Chromatography, High Pressure Liquid Chromatography, Thin Layer Fluorescent Antibody Technique, Indirect Galactose Glycolipids / chemistry*, immunology, metabolism* Immunoglobulin Fab Fragments / immunology Leishmania major / growth & development, pathogenicity* Leishmaniasis, Cutaneous / parasitology Macrophages, Peritoneal / parasitology* Mice Mice, Inbred BALB C Phosphatidylinositols / chemistry, immunology |
| Chemical | |
Reg. No./Substance:
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0/Antibodies, Monoclonal; 0/Antibodies, Protozoan; 0/Glycolipids; 0/Immunoglobulin Fab Fragments; 0/Phosphatidylinositols; 26566-61-0/Galactose |
| Comments/Corrections | |
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