Document Detail

Role of beta-D-galactofuranose in Leishmania major macrophage invasion.
MedLine Citation:
PMID:  12438330     Owner:  NLM     Status:  MEDLINE    
The role of glycosylinositol phospholipid 1 (GIPL-1) of Leishmania (Leishmania) major in the interaction of promastigotes and amastigotes with macrophages was analyzed. Monoclonal antibody MEST-1, which recognizes glycolipids containing terminal galactofuranose (Galf) residues (E. Suzuki, M. S. Toledo, H. K. Takahashi, and A. H. Straus, Glycobiology 7:463-468, 1997), was used to detect GIPL-1 in Leishmania by indirect immunofluorescence and to analyze its role in macrophage infectivity. L. major promastigotes showed intense fluorescence with MEST-1, and GIPL-1 was detected in both amastigote and promastigote forms by high-performance thin-layer chromatography immunostaining by using MEST-1. Delipidation of L. major promastigotes with isopropanol-hexane-water eliminated the MEST-1 reactivity, confirming that only GIPL-1 is recognized in either amastigotes or promastigotes of this species. The biological role of GIPL-1 in the ability of L. major to invade macrophages was studied by using either Fab fragments of MEST-1 or methylglycosides. Preincubation of parasites with Fab fragments reduced macrophage infectivity in about 80% of the promastigotes and 30% of the amastigotes. Preincubation of peritoneal macrophages with p-nitrophenyl-beta-galactofuranoside (10 mM) led to significant ( approximately 80%) inhibition of promastigote infectivity. These data suggest that a putative new receptor recognizing beta-D-Galf is associated with L. major macrophage infectivity and that GIPL-1 containing a terminal Galf residue is involved in the L. major-macrophage interaction.
Erika Suzuki; Ameria K Tanaka; Marcos S Toledo; Helio K Takahashi; Anita H Straus
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Infection and immunity     Volume:  70     ISSN:  0019-9567     ISO Abbreviation:  Infect. Immun.     Publication Date:  2002 Dec 
Date Detail:
Created Date:  2002-11-19     Completed Date:  2003-01-07     Revised Date:  2013-04-18    
Medline Journal Info:
Nlm Unique ID:  0246127     Medline TA:  Infect Immun     Country:  United States    
Other Details:
Languages:  eng     Pagination:  6592-6     Citation Subset:  IM    
Department of Biochemistry, Universidade Federal de São Paulo/Escola Paulista de Medicina, Brazil.
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MeSH Terms
Antibodies, Monoclonal / immunology*
Antibodies, Protozoan / immunology*
Chromatography, High Pressure Liquid
Chromatography, Thin Layer
Fluorescent Antibody Technique, Indirect
Glycolipids / chemistry*,  immunology,  metabolism*
Immunoglobulin Fab Fragments / immunology
Leishmania major / growth & development,  pathogenicity*
Leishmaniasis, Cutaneous / parasitology
Macrophages, Peritoneal / parasitology*
Mice, Inbred BALB C
Phosphatidylinositols / chemistry,  immunology
Reg. No./Substance:
0/Antibodies, Monoclonal; 0/Antibodies, Protozoan; 0/Glycolipids; 0/Immunoglobulin Fab Fragments; 0/Phosphatidylinositols; 26566-61-0/Galactose

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