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Role of bacterial isolates in enhancing the bud induction in the industrially important red alga Gracilaria dura.
MedLine Citation:
PMID:  21255055     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
Plant growth depends on the integration of environmental cues, nitrogen fixation and phytohormone-signaling pathways. The growth and development of Gracilaria dura was significantly influenced by the association of bacterial isolates. The putative bud-inducing epiphytic Exiguobacterium homiense and endophytic Bacillus pumilus, B. licheniformis were examined for their ability to fix nitrogen and produce indole-3-acetic acid. These bacterial isolates were identified to the species level by biochemical tests, fatty acid and partial 16S rRNA gene sequence analysis. The B. pumilus, B. licheniformis and E. homiense produced 445.5, 335 and 184.1 μg mL(-1) indole-3-acetic acid and 12.51, 10.14 and 6.9 mM mL(-1) ammonium respectively as determined with HPLC and spectroscopy. New bud regeneration observed after the addition of total protein of the bacterial isolates suggests that IAA is conjugated with protein. The epi and endophytic bacterial isolates were able to induce 5 and 10 new buds per frond respectively as comparison to control where 1-2 buds were observed. The combination of 25°C and 30‰ showed optimum condition for bud induction in G. dura when incubated with the total protein of B. pumilus. Our finding for the first time revealed that IAA coupled with nitrogen fixation induce and regenerate new buds in G. dura.
Authors:
Ravindra Pal Singh; A J Bijo; Ravi S Baghel; C R K Reddy; Bhavanath Jha
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Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2011-1-21
Journal Detail:
Title:  FEMS microbiology ecology     Volume:  -     ISSN:  1574-6941     ISO Abbreviation:  -     Publication Date:  2011 Jan 
Date Detail:
Created Date:  2011-1-24     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8901229     Medline TA:  FEMS Microbiol Ecol     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Copyright Information:
© 2011 Federation of European Microbiological Societies. Published by Blackwell Publishing Ltd. All rights reserved.
Affiliation:
Discipline of Marine Biotechnology and Ecology, Central Salt and Marine Chemicals Research Institute, Council of Scientific and Industrial Research (CSIR), Bhavnagar 364021, India.
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