Document Detail


Role of astrocytic leptin receptor subtypes on leptin permeation across hCMEC/D3 human brain endothelial cells.
MedLine Citation:
PMID:  20977476     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Astrocytic leptin receptors (ObR) can be up-regulated in conditions such as adult-onset obesity. To determine whether the levels and subtypes of astrocytic ObR modulate leptin transport, we co-cultured hCMEC/D3 human brain endothelial cells and C6 astrocytoma cells in the Transwell system, and tested leptin permeation from apical to basolateral chambers. In comparison with hCMEC alone, co-culture of C6 cells reduced the permeability of paracellular markers and leptin. Unexpectedly, ObRb over-expression in C6 cells increased leptin permeation whereas ObRa over-expression showed no effect when compared with the control group of pcDNA-transfected C6 cells. By contrast, the paracellular permeability to the sodium fluorescein control was unchanged by over-expression of ObR subtypes. Leptin remained intact after crossing the monolayer as shown by HPLC and acid precipitation, and this was not affected by C6 cell co-culture or the over-expression of different ObR subtypes. Thus, increased expression of ObRb (and to a lesser extent ObRe) in C6 cells specifically increased the permeation of leptin across the hCMEC monolayer. Consistent with the evidence that the most apparent regulatory changes of ObR during obesity and inflammation occur in astrocytes, the results indicate that astrocytes actively regulate leptin transport across the blood-brain barrier, a mechanism independent of reduction of paracellular permeability.
Authors:
Hung Hsuchou; Abba J Kastin; Hong Tu; N Joan Abbott; Pierre-Olivier Couraud; Weihong Pan
Related Documents :
19429046 - Enteric glial cells express full-length trkb and depend on trkb expression for normal d...
11556886 - Rat microglial cells secrete predominantly the precursor of interleukin-1beta in respon...
19915696 - Ape1/ref-1 stimulates gdnf/gfralpha1-mediated downstream signaling and neuroblastoma pr...
7562616 - Tyrosine phosphorylation during synapse formation between identified leech neurons.
16502256 - The neuropeptide fmrfamide can protect cells against apoptosis in the snail digestive g...
8918946 - Effect of multiplicity of infection on listeria monocytogenes pathogenicity for hela an...
Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural     Date:  2010-10-26
Journal Detail:
Title:  Journal of neurochemistry     Volume:  115     ISSN:  1471-4159     ISO Abbreviation:  J. Neurochem.     Publication Date:  2010 Dec 
Date Detail:
Created Date:  2010-11-03     Completed Date:  2010-11-29     Revised Date:  2014-09-22    
Medline Journal Info:
Nlm Unique ID:  2985190R     Medline TA:  J Neurochem     Country:  England    
Other Details:
Languages:  eng     Pagination:  1288-98     Citation Subset:  IM    
Copyright Information:
© 2010 The Authors. Journal of Neurochemistry © 2010 International Society for Neurochemistry.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:
Albumins / metabolism
Animals
Astrocytes / drug effects,  metabolism*
Biological Transport / drug effects
Brain / cytology
Cell Line, Transformed
Chromatography, High Pressure Liquid / methods
Coculture Techniques
Endothelial Cells / drug effects,  metabolism*
Fluorescein-5-isothiocyanate / metabolism
Humans
Iodine Isotopes / metabolism
Leptin / metabolism*,  pharmacology
Mice
Receptors, Leptin / genetics,  physiology*
Rhodamines / metabolism
Time Factors
Transfection / methods
Up-Regulation / physiology
Grant Support
ID/Acronym/Agency:
DK54880/DK/NIDDK NIH HHS; NS62291/NS/NINDS NIH HHS; R01 DK054880/DK/NIDDK NIH HHS; R01 DK092245/DK/NIDDK NIH HHS; R01 NS062291/NS/NINDS NIH HHS; R01 NS062291-04/NS/NINDS NIH HHS; R56 DK054880/DK/NIDDK NIH HHS; R56 DK054880-11/DK/NIDDK NIH HHS
Chemical
Reg. No./Substance:
0/Albumins; 0/Iodine Isotopes; 0/Leptin; 0/Receptors, Leptin; 0/Rhodamines; 4158-89-8/tetramethylrhodamine isothiocyanate; I223NX31W9/Fluorescein-5-isothiocyanate
Comments/Corrections

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Methodological considerations for analyzing trabecular architecture: an example from the primate han...
Next Document:  Progesterone inhibits estrogen-mediated neuroprotection against excitotoxicity by down-regulating es...