| Role of aromatic amino acids in protein-nucleic acid recognition. | |
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MedLine Citation:
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PMID: 17219397 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Statistical analysis of structures from the PBD has been used to examine the role that the aromatic amino acids play in protein-nucleic acid recognition. In protein-DNA complexes, the residues Phe and His are found to bind selectively to the DNA chain--Phe to A and T, and His to T and G. The preferred binding modes are identified, and the interactions involving Phe are shown to be important in the transcription process. In protein-RNA complexes, Phe is found to occur far less often and is instead replaced by Trp, which binds selectively to C and G, offering a possible mechanism for differentiation between the two nucleic acids. SASA analysis of the two sets of complexes suggests that all of the aromatic amino acids are more heavily involved in binding than would be expected on the balance of probability. Phe and Tyr occur approximately equal in both sets of data, whereas the proportions of His and Trp vary considerably, supporting the idea that these residues may be involved in differentiating between the two nucleic acids. |
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Authors:
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Christopher M Baker; Guy H Grant |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Biopolymers Volume: 85 ISSN: 0006-3525 ISO Abbreviation: Biopolymers Publication Date: 2007 Apr 5-15 |
Date Detail:
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Created Date: 2007-03-27 Completed Date: 2007-07-30 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 0372525 Medline TA: Biopolymers Country: United States |
Other Details:
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Languages: eng Pagination: 456-70 Citation Subset: IM |
Affiliation:
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Department of Chemistry, Physical and Theoretical Chemistry Laboratory, University of Oxford, South Parks Road, Oxford OX1 3QZ, UK. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Amino Acids, Aromatic
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chemistry*,
metabolism Binding Sites DNA / chemistry, metabolism DNA-Binding Proteins / chemistry*, metabolism Data Interpretation, Statistical Hydrogen Bonding Models, Molecular Protein Binding RNA / chemistry, metabolism RNA-Binding Proteins / chemistry*, metabolism |
| Chemical | |
Reg. No./Substance:
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0/Amino Acids, Aromatic; 0/DNA-Binding Proteins; 0/RNA-Binding Proteins; 63231-63-0/RNA; 9007-49-2/DNA |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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