| Role of angiotensin II-mediated AMPK inactivation on obesity-related salt-sensitive hypertension. | |
| | |
MedLine Citation:
|
PMID: 22293193 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
|
Salt-sensitive hypertension is a characteristic of the metabolic syndrome. Given the links to cardiovascular events, the mechanisms underlying sodium metabolism may represent an important therapeutic target for this disorder. Angiotensin II (AII) is a key peptide underlying sodium retention. However, 5'AMP-activated protein kinase (AMPK) has also been reported to participate in the regulation of ion transport. In this study we examined the relationship between AII and AMPK on the development of hypertension in two salt-sensitive mouse models. In the first model, the mice were maintained on a high-fat diet (HFD) for 12weeks, in order to develop features similar to the metabolic syndrome, including salt-sensitive hypertension. HFD-induced obese mice showed elevated systolic blood pressure and lower sodium excretion in response to salt loading, along with an increase in AII contents and inactivation of AMPK in the kidney, which were significantly improved by the treatment of an angiotensin II antagonist, losartan, for 2weeks. To clarify the effects of AII, a second group of mice was infused with AII via an osmotic pump, which led to higher systolic blood pressure, and decreases in urinary sodium excretion and the expression of AMPK, in a manner similar to those observed in the HFD mice. However, treatment with an AMPK activator, metformin, improved the changes induced by the AII, suggesting that AII induced sodium retention works by acting on AMPK activity. Finally, we evaluated the changes in salt-sensitivity by performing 2-week salt loading experiments with or without metformin. AII infusion elevated blood pressure by salt loading but metformin prevented it. These findings indicate that AII suppresses AMPK activity in the kidney, leading to sodium retention and enhanced salt-sensitivity, and that AMPK activation may represent a new therapeutic target for obesity-related salt-sensitive hypertension. |
| | |
Authors:
|
Naoko Deji; Shinji Kume; Shin-Ichi Araki; Keiji Isshiki; Hisazumi Araki; Masami Chin-Kanasaki; Yuki Tanaka; Akira Nishiyama; Daisuke Koya; Masakazu Haneda; Atsunori Kashiwagi; Hiroshi Maegawa; Takashi Uzu |
Related Documents
:
|
21585643 - Rapid versus stepwise application of negative pressure in vacuum extraction-assisted va... 21552063 - Anal canal vector volume manometry. 18174783 - Prompt, aggressive bp lowering in high-risk patients. |
Publication Detail:
|
Type: JOURNAL ARTICLE Date: 2012-1-24 |
Journal Detail:
|
Title: Biochemical and biophysical research communications Volume: - ISSN: 1090-2104 ISO Abbreviation: - Publication Date: 2012 Jan |
Date Detail:
|
Created Date: 2012-2-1 Completed Date: - Revised Date: - |
Medline Journal Info:
|
Nlm Unique ID: 0372516 Medline TA: Biochem Biophys Res Commun Country: - |
Other Details:
|
Languages: ENG Pagination: - Citation Subset: - |
Copyright Information:
|
Copyright © 2012. Published by Elsevier Inc. |
Affiliation:
|
Department of Medicine, Shiga University of Medical Science, Otsu, Shiga, Japan. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
|
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Protein kinase A phosphorylates Down syndrome critical region 1 (RCAN1).
Next Document: Functional analysis of the rice rubisco activase promoter in transgenic Arabidopsis.