Document Detail


Role of alphavbeta3 integrin receptor in the invasive potential of human cervical cancer (SiHa) cells.
MedLine Citation:
PMID:  11797830     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
One of the most important cell surface receptors in tumor development is alphavbeta3. To study the role of the alphavbeta3 integrin receptor in the invasive properties of tumor cells, we used human cervical tumor cells SiHa (cell surface alphavbeta3 integrin receptor-positive) and HeLa cells (cell surface alphavbeta3 integrin receptor-negative). Cell adhesion assay showed that SiHa and HeLa cells can bind very efficiently to extracellular matrix proteins fibronectin, laminin, and collagen IV, but the binding of HeLa cells to vitronectin is very poor compared to that of SiHa cells. Comparative invasion assay demonstrated a much lower invasive potential of HeLa cells than SiHa cells. Cell surface alphav and beta3 integrin receptor subunit assay showed the expression of alphavbeta3 integrin receptor on the SiHa cell surface, whereas the HeLa cell surface lacks functional alphavbeta3 heterodimer. The zymogram demonstrated a higher gelatinase/MMP-2 activity in culture medium, whole cell, and membrane extract of SiHa cells than that in HeLa cells. The alphavbeta3 integrin receptor-associated MMP-2 activity of SiHa and HeLa cells was tested in a comparative zymography that clearly showed very high gelatinase/MMP-2 activity in alphav mAb-immunoprecipitated fraction of SiHa cell (containing alphavbeta3 heterodimer) but not in the alphav mAb-immunoprecipitated fraction of HeLa cell membrane extract (containing only the beta3 subunit). Immunoblot assay of alphav monoclonal antibody-immunoprecipitated alphavbeta3 integrin receptor from SiHa cell membrane extract with MMP-2 monoclonal antibody demonstrated the association of MMP-2 protein with alphavbeta3 integrin receptor. We concluded that alphavbeta3 integrin receptor is one of the most important cell surface molecules regulating the invasive property of cervical tumor cells because of its associated gelatinase/MMP-2 activity. Our findings will contribute to a better understanding of the role of integrin receptors, especially of the alphavbeta3 integrin receptor, in the invasive property of cancer cells and possibly affect future therapeutic approaches to cancer invasion and metastasis.
Authors:
N Chatterjee; A Chatterjee
Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Journal of environmental pathology, toxicology and oncology : official organ of the International Society for Environmental Toxicology and Cancer     Volume:  20     ISSN:  0731-8898     ISO Abbreviation:  J. Environ. Pathol. Toxicol. Oncol.     Publication Date:  2001  
Date Detail:
Created Date:  2002-01-18     Completed Date:  2002-01-31     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  8501420     Medline TA:  J Environ Pathol Toxicol Oncol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  211-21     Citation Subset:  IM    
Affiliation:
Department of Receptor Biology and Tumor Metastasis, Chittaranjan National Cancer Institute, Calcutta, India.
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MeSH Terms
Descriptor/Qualifier:
Cell Membrane / physiology
Female
Gene Expression Regulation, Neoplastic*
Hela Cells / physiology
Humans
Immunoblotting
Matrix Metalloproteinase 2 / metabolism*
Neoplasm Invasiveness / physiopathology*
Neoplasm Metastasis / physiopathology*
Receptors, Vitronectin / biosynthesis,  physiology*
Uterine Cervical Neoplasms / physiopathology*
Chemical
Reg. No./Substance:
0/Receptors, Vitronectin; EC 3.4.24.24/Matrix Metalloproteinase 2

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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