Document Detail


Role of adhesins and toxins in invasion of human tracheal epithelial cells by Bordetella pertussis.
MedLine Citation:
PMID:  10722585     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Bordetella pertussis, the agent of whooping cough, can invade and survive in several types of eukaryotic cell, including CHO, HeLa 229, and HEp-2 cells and macrophages. In this study, we analyzed bacterial invasiveness in nonrespiratory human HeLa epithelial cells and human HTE and HAE0 tracheal epithelial cells. Invasion assays and transmission electron microscopy analysis showed that B. pertussis strains invaded and survived, without multiplying, in HTE or HAE0 cells. This phenomenon was bvg regulated, but invasive properties differed between B. pertussis strains and isolates and the B. pertussis reference strain. Studies with B. pertussis mutant strains demonstrated that filamentous hemagglutinin, the major adhesin, was involved in the invasion of human tracheal epithelial cells by bacteria but not in that of HeLa cells. Fimbriae and pertussis toxin were not found to be involved. However, we found that the production of adenylate cyclase-hemolysin prevents the invasion of HeLa and HTE cells by B. pertussis because an adenylate cyclase-hemolysin-deficient mutant was found to be more invasive than the parental strain. The effect of adenylate cyclase-hemolysin was mediated by an increase in the cyclic AMP concentration in the cells. Pertactin (PRN), an adhesin, significantly inhibited the invasion of HTE cells by bacteria, probably via its interaction with adenylate cyclase-hemolysin. Isolates producing different PRNs were taken up similarly, indicating that the differences in the sequences of the PRNs produced by these isolates do not affect invasion. We concluded that filamentous hemagglutinin production favored invasion of human tracheal cells but that adenylate cyclase-hemolysin and PRN production significantly inhibited this process.
Authors:
L Bassinet; P Gueirard; B Maitre; B Housset; P Gounon; N Guiso
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Infection and immunity     Volume:  68     ISSN:  0019-9567     ISO Abbreviation:  Infect. Immun.     Publication Date:  2000 Apr 
Date Detail:
Created Date:  2000-04-20     Completed Date:  2000-04-20     Revised Date:  2013-04-17    
Medline Journal Info:
Nlm Unique ID:  0246127     Medline TA:  Infect Immun     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  1934-41     Citation Subset:  IM    
Affiliation:
Laboratoire des Bordetella, Institut Pasteur, Paris, France.
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MeSH Terms
Descriptor/Qualifier:
Adenylate Cyclase Toxin
Adhesins, Bacterial / physiology*
Bacterial Outer Membrane Proteins / physiology
Bacterial Proteins / physiology
Bordetella pertussis / pathogenicity*
Cell Division
Cell Line, Transformed
Cyclic AMP / metabolism
Epithelial Cells / microbiology,  ultrastructure
HeLa Cells
Hemagglutinins / physiology
Humans
Microscopy, Electron
Pertussis Toxin
Protein Precursors / physiology
Time Factors
Toxins, Biological / metabolism*
Trachea / microbiology*
Virulence Factors, Bordetella / metabolism
Chemical
Reg. No./Substance:
0/Adenylate Cyclase Toxin; 0/Adhesins, Bacterial; 0/Bacterial Outer Membrane Proteins; 0/Bacterial Proteins; 0/Hemagglutinins; 0/Protein Precursors; 0/Toxins, Biological; 0/Virulence Factors, Bordetella; 0/filamentous hemagglutinin adhesin, Bordetella pertussis; 0/pertactin; 60-92-4/Cyclic AMP; EC 2.4.2.31/Pertussis Toxin
Comments/Corrections

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