| Role of adenosine receptors in volatile anesthetic preconditioning against neutrophil-induced contractile dysfunction in isolated rat hearts. | |
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MedLine Citation:
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PMID: 16052111 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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BACKGROUND: The authors tested the hypothesis that adenosine receptors in polymorphonuclear neutrophils and the heart mediate the preconditioning effects of volatile anesthetics against neutrophil-induced contractile dysfunction. METHODS: Studies were conducted in buffer-perfused and paced isolated rat hearts. Left ventricular developed pressure served as index of contractility. Neutrophils and platelet-activating factor were added to perfusate for 10 min followed by 30 min of recovery. The effect of selective pretreatment of the neutrophils and the hearts with 1.0 minimum alveolar concentration isoflurane or sevoflurane on the neutrophil-induced contractile dysfunction was assessed. Studies were performed in the absence and presence of the nonselective adenosine receptor antagonist 8-phenyltheophylline (10 microM). Neutrophil retention was determined from difference between those administered and collected in coronary effluent and from myeloperoxidase concentration in myocardial samples. Superoxide production of neutrophils was measured by spectrophotometry. RESULTS: Under control conditions (no anesthetic pretreatment), activated neutrophils caused marked and persistent reductions in left ventricular developed pressure, associated with increases in neutrophil retention and myeloperoxidase activity. Pretreatment of the neutrophils or the heart with either isoflurane or sevoflurane abolished these effects. Pretreatment of the neutrophils also reduced the platelet-activating factor-induced increase in superoxide production by 29 and 33%, respectively. 8-Phenyltheophylline blunted the effects of anesthetic pretreatment of the neutrophils, whereas it did not alter the effects of anesthetic pretreatment of the heart. CONCLUSION: An activation of adenosine receptors in neutrophils, but not in the heart, plays a role in the preconditioning effects of volatile anesthetics against neutrophil-induced contractile dysfunction. |
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Authors:
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Guochang Hu; M Ramez Salem; George J Crystal |
Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Anesthesiology Volume: 103 ISSN: 0003-3022 ISO Abbreviation: Anesthesiology Publication Date: 2005 Aug |
Date Detail:
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Created Date: 2005-07-29 Completed Date: 2005-09-08 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 1300217 Medline TA: Anesthesiology Country: United States |
Other Details:
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Languages: eng Pagination: 287-95 Citation Subset: AIM; IM |
Affiliation:
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Department of Anesthesiology, Advocate Illinois Masonic Medical Center, Chicago, Illinois 60657-5193, USA. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Anesthetics, Inhalation
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pharmacology* Animals Dogs Female Ischemic Preconditioning, Myocardial* Male Myocardial Contraction / drug effects* Neutrophils / drug effects, physiology* Peroxidase / metabolism Potassium Channels / drug effects Rats Rats, Sprague-Dawley Receptors, Purinergic P1 / physiology* Superoxides / metabolism Ventricular Function, Left / drug effects |
| Chemical | |
Reg. No./Substance:
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0/Anesthetics, Inhalation; 0/Potassium Channels; 0/Receptors, Purinergic P1; 11062-77-4/Superoxides; EC 1.11.1.7/Peroxidase |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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