Document Detail


Role of activin-A and myostatin and their signaling pathway in human myometrial and leiomyoma cell function.
MedLine Citation:
PMID:  24606069     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
Context: Uterine leiomyomas are highly prevalent benign tumors of pre-menopausal women and the most common indication for hysterectomy. However, the exact etiology of this tumor is not fully understood. Objective: To evaluate the role of activin-A and myostatin, and their signaling pathways in human myometrial and leiomyoma cells. Design: Laboratory study Setting: Myometrial and leiomyoma cells (primary and cell lines) were cultured in vitro. Patients: The study included premenopausal women who were admitted to the hospital for myomectomy or hysterectomy. Interventions: Primary myometrial and leiomyoma cells and/or cell lines were treated with activin-A (4nM) and myostatin (4nM) for different days of interval (to measure proliferation rate) or 30 min (to measure signaling molecules) or 48 h to measure proliferating markers, extracellular matrix mRNA and/or protein expression by real-time polymerase chain reaction (PCR), western blot and/or immunocytochemistry. Results: We found that activin-A and myostatin significantly reduce cell proliferation in primary myometrial cells but not in leiomyoma cells as measured by CyQUANT cell proliferation assay kit. Reduced expression of PCNA and Ki-67 were also observed in myometrial cells in response to activin-A and myostatin treatment. Activin-A also significantly increased mRNA expression of fibronectin, collagen1A1 and versican in primary leiomyoma cells. Finally, we found that activin-A and myostatin activate Smad2/3 signaling but do not affect Erk or p38 signaling in both myometrial and leiomyoma cells. Conclusions: This study results suggest that activin-A and myostatin can exert antiproliferative and/or fibrotic effects on these cell types via Smad2/3 signaling.
Authors:
Md Soriful Islam; William H Catherino; Olga Protic; Milijana Janjusevic; Peter Clarke Gray; Stefano Raffaele Giannubilo; Andrea Ciavattini; Pasquale Lamanna; Andrea Luigi Tranquilli; Felice Petraglia; Mario Castellucci; Pasquapina Ciarmela
Related Documents :
24484729 - Vertical nanowire probes for intracellular signaling of living cells.
24104579 - Nano-photonic light trapping near the lambertian limit in organic solar cell architectu...
23529159 - Use of the tetrazolium salt mtt to measure cell viability effects of the bacterial anta...
24960549 - Synthesis and evaluation of bisbenzylidenedioxotetrahydrothiopranones as activators of ...
24429989 - Chitin synthetase from yeast-type cells ofmucor rouxii induced by malonic acid: a scree...
23527039 - Real-time motion analysis reveals cell directionality as an indicator of breast cancer ...
19782669 - Dynamics of caspase-3-mediated apoptosis during spinal cord regeneration in the teleost...
2545549 - Detection of oxygen radicals during reperfusion of intestinal cells in vitro.
9133319 - Requirement of a large k+-uptake capacity and of extracytoplasmic protease activity for...
Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2014-2-25
Journal Detail:
Title:  The Journal of clinical endocrinology and metabolism     Volume:  -     ISSN:  1945-7197     ISO Abbreviation:  J. Clin. Endocrinol. Metab.     Publication Date:  2014 Feb 
Date Detail:
Created Date:  2014-3-10     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0375362     Medline TA:  J Clin Endocrinol Metab     Country:  -    
Other Details:
Languages:  ENG     Pagination:  jc20132623     Citation Subset:  -    
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Peripheral monocytes of obese women display increased chemokine receptor expression and migration ca...
Next Document:  Acute and short term chronic testosterone fluctuations effects on glucose homeostasis, insulin sensi...