| Role of acidic sphingomyelinase in thymol-mediated dendritic cell death. | |
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MedLine Citation:
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PMID: 20603831 Owner: NLM Status: In-Process |
Abstract/OtherAbstract:
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SCOPE: Thymol is a component of several plants with antimicrobial activity. Little is known about the effects of thymol on immune cells of the host. This study addressed the effects of thymol on dendritic cells (DCs), regulators of innate and adaptive immunity. METHODS AND RESULTS: Immunohistochemistry, Western blotting and fluorescence-activated cell sorting analysis were performed in bone marrow-derived DCs either from wild-type mice or from mice lacking acid sphingomyelinase (ASM⁻/⁻) treated and untreated for 24 h with thymol (2-100 μg/mL). Thymol treatment resulted in activation of ASM, stimulation of ceramide formation, downregulation of anti-apoptotic Bcl-2 and Bcl-xL proteins, activation of caspase 3 and caspase 8, DNA fragmentation as well as cell membrane scrambling. The effects were dependent on the presence of ASM and were lacking in ASM⁻/⁻ mice or in wild-type DCs treated with sphingomyelinase inhibitor amitriptyline. CONCLUSION: Thymol triggers suicidal DC death, an effect mediated by and requiring activation of ASM. |
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Authors:
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Nguyen Thi Xuan; Ekaterina Shumilina; Evi Schmid; Shefalee K Bhavsar; Rexhep Rexhepaj; Friedrich Götz; Erich Gulbins; Florian Lang |
Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Molecular nutrition & food research Volume: 54 ISSN: 1613-4133 ISO Abbreviation: Mol Nutr Food Res Publication Date: 2010 Dec |
Date Detail:
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Created Date: 2010-12-02 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 101231818 Medline TA: Mol Nutr Food Res Country: Germany |
Other Details:
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Languages: eng Pagination: 1833-41 Citation Subset: IM |
Copyright Information:
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Copyright © 2010 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim. |
Affiliation:
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Department of Physiology, University of Tübingen, Tübingen, Germany. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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