Document Detail

Role of acetate in production of an autoinducible class IIa bacteriocin in Carnobacterium piscicola A9b.
MedLine Citation:
PMID:  11976095     Owner:  NLM     Status:  MEDLINE    
Carnobacterium piscicola strain A9b isolated from cold smoked salmon inhibits growth of the food-borne pathogen Listeria monocytogenes partly due to the production of a proteinaceous compound (L. Nilsson, L. Gram, and H. H. Huss. J. Food Prot. 62:336-342, 1999). The purpose of the present study was to purify the compound and describe factors affecting its production, with particular emphasis on food-relevant factors. Amino acid sequencing showed that the compound is a class IIa bacteriocin with an N-terminal amino acid sequence identical to that of carnobacteriocin B2. The production of the bacteriocin was autoinducible, and the threshold level for induction was 9.6 x 10(-10) M. We also report, for the first time, that acetate acts as an induction factor, with a threshold concentration of 0.3 to 12 mM. Acetate could not act as an inducer during the late exponential phase of C. piscicola A9b. The induction of bacteriocin production showed a dose-dependent relationship at acetate concentrations of up to 10 to 20 mM (depending on the growth medium) and at a concentration of 1.9 x 10(-8) M for the bacteriocin itself; a saturation level of bacteriocin specific activity was reached at these concentrations of induction factors. The combined use of both inducers did not enhance the saturation level of bacteriocin production compared to that seen with the use of each inducer alone. Increasing NaCl and glucose concentrations negatively influenced the efficiency of acetate as an induction factor. Based on the results, carnobacteriocin B2 was used as an induction factor to manipulate the production of bacteriocin in cold smoked salmon juice and thus improve the ability to inhibit L. monocytogenes.
Lilian Nilsson; Michael K Nielsen; Yin Ng; Lone Gram
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Applied and environmental microbiology     Volume:  68     ISSN:  0099-2240     ISO Abbreviation:  Appl. Environ. Microbiol.     Publication Date:  2002 May 
Date Detail:
Created Date:  2002-04-26     Completed Date:  2002-07-12     Revised Date:  2013-06-09    
Medline Journal Info:
Nlm Unique ID:  7605801     Medline TA:  Appl Environ Microbiol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  2251-60     Citation Subset:  IM    
Danish Institute for Fisheries Research, Department of Seafood Research, c/o Technical University of Denmark, DK-2800 Lyngby, Denmark.
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MeSH Terms
Acetic Acid / pharmacology*
Bacterial Proteins / biosynthesis
Bacteriocins / biosynthesis,  chemistry,  isolation & purification,  metabolism*
Clostridium / drug effects*,  metabolism
Culture Media
Dose-Response Relationship, Drug
Drug Interactions
Drug Stability
Glucose / pharmacology
Sequence Analysis, Protein
Reg. No./Substance:
0/Bacterial Proteins; 0/Bacteriocins; 0/Culture Media; 155982-38-0/bacteriocin B2 protein, Carnobacterium piscicola; 50-99-7/Glucose; 64-19-7/Acetic Acid

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