| Role of ZNF143 in tumor growth through transcriptional regulation of DNA replication and cell-cycle-associated genes. | |
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MedLine Citation:
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PMID: 20860770 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The cell cycle is strictly regulated by numerous mechanisms to ensure cell division. The transcriptional regulation of cell-cycle-related genes is poorly understood, with the exception of the E2F family that governs the cell cycle. Here, we show that a transcription factor, zinc finger protein 143 (ZNF143), positively regulates many cell-cycle-associated genes and is highly expressed in multiple solid tumors. RNA-interference (RNAi)-mediated knockdown of ZNF143 showed that expression of 152 genes was downregulated in human prostate cancer PC3 cells. Among these ZNF143 targets, 41 genes (27%) were associated with cell cycle and DNA replication including cell division cycle 6 homolog (CDC6), polo-like kinase 1 (PLK1) and minichromosome maintenance complex component (MCM) DNA replication proteins. Furthermore, RNAi of ZNF143 induced apoptosis following G2/M cell cycle arrest. Cell growth of 10 lung cancer cell lines was significantly correlated with cellular expression of ZNF143. Our data suggest that ZNF143 might be a master regulator of the cell cycle. Our findings also indicate that ZNF143 is a member of the growing list of non-oncogenes that are promising cancer drug targets. |
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Authors:
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Hiroto Izumi; Tetsuro Wakasugi; Shohei Shimajiri; Akihide Tanimoto; Yasuyuki Sasaguri; Eiji Kashiwagi; Yoshihiro Yasuniwa; Masaki Akiyama; Bin Han; Ying Wu; Takeshi Uchiumi; Tokuzo Arao; Kazuto Nishio; Ryuta Yamazaki; Kimitoshi Kohno |
Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2010-09-23 |
Journal Detail:
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Title: Cancer science Volume: 101 ISSN: 1349-7006 ISO Abbreviation: Cancer Sci. Publication Date: 2010 Dec |
Date Detail:
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Created Date: 2010-11-16 Completed Date: 2010-12-15 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 101168776 Medline TA: Cancer Sci Country: England |
Other Details:
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Languages: eng Pagination: 2538-45 Citation Subset: IM |
Copyright Information:
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© 2010 Japanese Cancer Association. |
Affiliation:
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Department of Molecular Biology Otorhinolaryngology Pathology and Cell Biology, School of Medicine, University of Occupational and Environmental Health, Kitakyushu, Japan. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Blotting, Western Cell Line, Tumor Cell Proliferation Cell Separation Chromatin Immunoprecipitation DNA Replication / physiology* Flow Cytometry Gene Expression Gene Expression Regulation, Neoplastic* Genes, cdc* Humans Immunohistochemistry Neoplasms / genetics*, metabolism Oligonucleotide Array Sequence Analysis RNA Interference Trans-Activators / metabolism* |
| Chemical | |
Reg. No./Substance:
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0/Trans-Activators; 0/ZNF143 protein, human |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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