Document Detail

Role of the transient receptor potential vanilloid type 1 channel in renal inflammation induced by lipopolysaccharide in mice.
MedLine Citation:
PMID:  23152109     Owner:  NLM     Status:  MEDLINE    
To determine the role of the transient receptor potential vanilloid type 1 (TRPV1) channel in the regulation of renal inflammation, lipopolysaccharide (LPS, 3 mg/kg) was intraperitoneally injected into wild-type (WT) and TRPV1-null mutant (TRPV1(-/-)) mice. The kidney and serum were collected 6 or 24 h after LPS injection for morphological analysis and proinflammatory cytokine assay. LPS injection led to a similar degree of transient hypotension and bradycardia in WT and TRPV1(-/-) mice determined by a telemetry system. LPS administration caused parenchymal red blood cell congestion and fading of intact glomerular structure in TRPV1(-/-) compared with WT mice. Serum creatinine levels were higher 24 h after LPS injection in TRPV1(-/-) than in WT mice. Neutrophil and macrophage infiltration in the kidneys was greater 6 h for the former and 24 h for both after LPS injection in TRPV1(-/-) than in WT mice. Serum cytokine levels including tumor necrosis factor (TNF)-α, IL-1β, and IL-6 were higher 6 h after LPS injection in TRPV1(-/-) compared with WT mice. Likewise, renal chemokine levels including keratinocyte-derived chemokines and macrophage inflammatory protein were higher 6 h after LPS injection in TRPV1(-/-) than in WT mice. Renal VCAM-1 and ICAM-1 expression was further elevated 6 h for the former and 24 h for the latter after LPS injection in TRPV1(-/-) than in WT mice. Renal nuclear factor-κB (NF-κB) activity was further increased 6 h after LPS injection in TRPV1(-/-) compared with WT mice. Pharmacological blockade TRPV1 in WT mice showed aggravated renal and serum inflammatory responses resembling that of TRPV1(-/-) mice. Thus TRPV1 gene ablation exacerbates LPS-induced renal tissue and function injury, including aggravated renal neutrophil and macrophage infiltration, chemokine and adhesion molecule levels, and glomerular hypercellularity accompanying with further increased serum creatinine and cytokine levels. These results indicate that TRPV1 is activated during LPS challenge, which may constitute a protect mechanism against LPS-induced renal injury via reducing renal inflammatory responses.
Youping Wang; Donna H Wang
Related Documents :
20834149 - Possible link of a compositional change in intestinal microbiota with the anti-allergic...
6479099 - Low levels of intestinal vitamin d-dependent calcium-binding protein in juvenile x-link...
22942429 - Lack of the lectin-like domain of thrombomodulin worsens shiga toxin-associated hemolyt...
12615699 - Suppression of intestinal polyposis in mdr1-deficient apcmin/+ mice.
6735079 - Gastric emptying and small intestinal transit in the piebald mouse model for hirschspru...
19907319 - Somatostatin and the gastrointestinal tract.
20385829 - Transgenic ablation of doublecortin-expressing cells suppresses adult neurogenesis and ...
16387789 - Surfactant protein d is proatherogenic in mice.
1940339 - Effects of recombinant transforming growth factor-beta 1 on hematologic recovery after ...
Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't     Date:  2012-11-14
Journal Detail:
Title:  American journal of physiology. Regulatory, integrative and comparative physiology     Volume:  304     ISSN:  1522-1490     ISO Abbreviation:  Am. J. Physiol. Regul. Integr. Comp. Physiol.     Publication Date:  2013 Jan 
Date Detail:
Created Date:  2013-01-02     Completed Date:  2013-03-07     Revised Date:  2014-01-09    
Medline Journal Info:
Nlm Unique ID:  100901230     Medline TA:  Am J Physiol Regul Integr Comp Physiol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  R1-9     Citation Subset:  IM    
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Bradycardia / chemically induced
Creatine / blood
Cytokines / analysis
Gene Silencing
Hypotension / chemically induced
Kidney / chemistry
Lipopolysaccharides / pharmacology
Mice, Inbred C57BL
Nephritis / chemically induced*,  pathology,  physiopathology*
TRPV Cation Channels / antagonists & inhibitors,  genetics,  physiology*
Grant Support
Reg. No./Substance:
0/Cytokines; 0/Lipopolysaccharides; 0/TRPV Cation Channels; 0/TRPV1 protein, mouse; MU72812GK0/Creatine

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Previous Document:  Seasonal restructuring of the ground squirrel gut microbiota over the annual hibernation cycle.
Next Document:  Effect of chronic perinatal hypoxia on the role of rho-kinase in pulmonary artery contraction in new...