| Role of the transient receptor potential vanilloid type 1 channel in renal inflammation induced by lipopolysaccharide in mice. | |
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MedLine Citation:
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PMID: 23152109 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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To determine the role of the transient receptor potential vanilloid type 1 (TRPV1) channel in the regulation of renal inflammation, lipopolysaccharide (LPS, 3 mg/kg) was intraperitoneally injected into wild-type (WT) and TRPV1-null mutant (TRPV1(-/-)) mice. The kidney and serum were collected 6 or 24 h after LPS injection for morphological analysis and proinflammatory cytokine assay. LPS injection led to a similar degree of transient hypotension and bradycardia in WT and TRPV1(-/-) mice determined by a telemetry system. LPS administration caused parenchymal red blood cell congestion and fading of intact glomerular structure in TRPV1(-/-) compared with WT mice. Serum creatinine levels were higher 24 h after LPS injection in TRPV1(-/-) than in WT mice. Neutrophil and macrophage infiltration in the kidneys was greater 6 h for the former and 24 h for both after LPS injection in TRPV1(-/-) than in WT mice. Serum cytokine levels including tumor necrosis factor (TNF)-α, IL-1β, and IL-6 were higher 6 h after LPS injection in TRPV1(-/-) compared with WT mice. Likewise, renal chemokine levels including keratinocyte-derived chemokines and macrophage inflammatory protein were higher 6 h after LPS injection in TRPV1(-/-) than in WT mice. Renal VCAM-1 and ICAM-1 expression was further elevated 6 h for the former and 24 h for the latter after LPS injection in TRPV1(-/-) than in WT mice. Renal nuclear factor-κB (NF-κB) activity was further increased 6 h after LPS injection in TRPV1(-/-) compared with WT mice. Pharmacological blockade TRPV1 in WT mice showed aggravated renal and serum inflammatory responses resembling that of TRPV1(-/-) mice. Thus TRPV1 gene ablation exacerbates LPS-induced renal tissue and function injury, including aggravated renal neutrophil and macrophage infiltration, chemokine and adhesion molecule levels, and glomerular hypercellularity accompanying with further increased serum creatinine and cytokine levels. These results indicate that TRPV1 is activated during LPS challenge, which may constitute a protect mechanism against LPS-induced renal injury via reducing renal inflammatory responses. |
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Authors:
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Youping Wang; Donna H Wang |
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Publication Detail:
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Type: Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't Date: 2012-11-14 |
Journal Detail:
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Title: American journal of physiology. Regulatory, integrative and comparative physiology Volume: 304 ISSN: 1522-1490 ISO Abbreviation: Am. J. Physiol. Regul. Integr. Comp. Physiol. Publication Date: 2013 Jan |
Date Detail:
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Created Date: 2013-01-02 Completed Date: 2013-03-07 Revised Date: 2013-04-16 |
Medline Journal Info:
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Nlm Unique ID: 100901230 Medline TA: Am J Physiol Regul Integr Comp Physiol Country: United States |
Other Details:
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Languages: eng Pagination: R1-9 Citation Subset: IM |
Affiliation:
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Central Laboratory and Division of Cardiology, First Affiliated Hospital, Henan University of Traditional Chinese Medicine, Zhengzhou, China. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Bradycardia / chemically induced Creatine / blood Cytokines / analysis Gene Silencing Hypotension / chemically induced Kidney / chemistry Lipopolysaccharides / pharmacology Macrophages Male Mice Mice, Inbred C57BL Nephritis / chemically induced*, pathology, physiopathology* Neutrophils TRPV Cation Channels / antagonists & inhibitors, genetics, physiology* |
| Grant Support | |
ID/Acronym/Agency:
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DK-67620/DK/NIDDK NIH HHS; HL-57853/HL/NHLBI NIH HHS; HL-73287/HL/NHLBI NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Cytokines; 0/Lipopolysaccharides; 0/TRPV Cation Channels; 0/TRPV1 protein, mouse; 57-00-1/Creatine |
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