| The role of transient receptor potential vanilloid 1 (TRPV1) receptors in dextran sulfate-induced colitis in mice. | |
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MedLine Citation:
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PMID: 20411352 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The aim of this study was to investigate the involvement of transient receptor potential vanilloid 1 (TRPV1) receptors in oral dextran sulfate sodium-induced (DSS) colitis using TRPV1 knockout mice and their wild-type C57BL/6 counterparts. DSS (2% or 5%) was administered orally ad libitum for 7 days; the controls received tap water. Animal weight, stool consistency, and blood content were scored every day to calculate the disease activity index (DAI). After sacrificing the mice on day 7, the colons were cut into three equal segments (proximal, intermediate, and distal) for histology, myeloperoxidase (MPO), and cytokine measurements. In the 2% DSS-treated group, the lack of TRPV1 receptors decreased the DAI. Each colon segment of wild-type animals showed more than two-fold increase of MPO activity and more severe histological changes compared to the knockouts. This difference was not observed in case of 5% DSS, when extremely severe inflammation occurred in both groups. IL-1beta production was not altered by the absence of TRPV1. In conclusion, activation of TRPV1 channels enhances the clinical symptoms, histopathological changes, and neutrophil accumulation induced by 2% DSS. Elucidating the modulator role of TRPV1 channels in inflammatory bowel diseases may contribute to the development of novel anti-inflammatory drugs for their therapy. |
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Authors:
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Istvan Szitter; Gabor Pozsgai; Katalin Sandor; Krisztian Elekes; Agnes Kemeny; Aniko Perkecz; Janos Szolcsanyi; Zsuzsanna Helyes; Erika Pinter |
Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2010-04-22 |
Journal Detail:
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Title: Journal of molecular neuroscience : MN Volume: 42 ISSN: 1559-1166 ISO Abbreviation: J. Mol. Neurosci. Publication Date: 2010 Sep |
Date Detail:
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Created Date: 2010-08-02 Completed Date: 2010-12-20 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 9002991 Medline TA: J Mol Neurosci Country: United States |
Other Details:
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Languages: eng Pagination: 80-8 Citation Subset: IM |
Affiliation:
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Department of Pharmacology and Pharmacotherapy, Faculty of Medicine, University of Pécs, 7624 Pécs, Szigeti u. 12, Hungary. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Colitis / chemically induced*, metabolism*, pathology Colon / drug effects, pathology Dextran Sulfate / pharmacology* Disease Models, Animal Dose-Response Relationship, Drug Female Inflammatory Bowel Diseases / metabolism, pathology Interleukin-1beta / metabolism Mice Mice, Inbred C57BL Mice, Knockout Peroxidase / metabolism TRPV Cation Channels / metabolism* |
| Chemical | |
Reg. No./Substance:
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0/Interleukin-1beta; 0/TRPV Cation Channels; 0/TRPV1 protein, mouse; 9042-14-2/Dextran Sulfate; EC 1.11.1.7/Peroxidase |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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