Document Detail

Role of thyroid hormones and mir-208 in myocardial remodeling in 5/6 nephrectomized rats.
MedLine Citation:
PMID:  24246300     Owner:  NLM     Status:  MEDLINE    
BACKGROUND AND AIMS: Thyroid hormones exert important effects on heart remodeling through mir-208. The process may have a role in myocardial changes in chronic kidney disease where thyroid abnormalities are common. In this study the effect of T4 supplementation on left ventricle (LV) remodeling in 5/6 nephrectomized rats (5/6Nx) was analyzed.
METHODS: 5/6Nx rats and 5/6Nx under T4 supplementation (5/6Nx + T4) were compared with control (C) and thyroidectomized (Tx) rats. After 8 weeks of follow-up, LV was analyzed for α-MHC, β-MHC, TGF-β, and mir-208 expression, hydroxyproline content, and myocardial fibrosis. Serum collagenase activity was also analyzed.
RESULTS: Heart weight increased in 5/6Nx rats compared to C, which was prevented with T4 supplementation (C, 1.5 ± 0.04; 5/6Nx, 1.8 ± 0.09; 5/6Nx + T4, 1.6 ± 0.07 g, p <0.05). The same pattern was seen for LV wall thickness, hydroxyproline content, LV fibrosis, and mRNA TGF-β expression (C, 0.47 ± 0.17; 5/6Nx, 10.55 ± 3.4; 5/6Nx + T4, 3.01 ± 0.52, p <0.01). Tx rats had reduction in heart weight, increased LV wall thickness, and fibrosis. Collagenase activity did not change in any group. mRNA expression of α-, β-MHC, and TGF-β increased in 5/6Nx in comparison to C and 5/6Nx + T4. Expression of mir-208 decreased in 5/6Nx groups, and levels were restored with T4 supplementation (4.21 ± 0.28, 3.39 ± 0.29, and 4.26 ± 0.37 RU, respectively, p <0.01).
CONCLUSIONS: Decreased plasma level of thyroid hormones or sensitivity at tissue level observed in chronic kidney disease induced by 5/6Nx has an important effect in heart remodeling processes, some of it related or mediated by mir-208 and TGF-β expression in the heart.
María-Del-Carmen Prado-Uribe; María-Virgilia Soto-Abraham; Carmen J Mora-Villalpando; Juan M Gallardo; Edmundo Bonilla; Marcela Avila; Eduardo Tena; Ramón Paniagua
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2013-11-15
Journal Detail:
Title:  Archives of medical research     Volume:  44     ISSN:  1873-5487     ISO Abbreviation:  Arch. Med. Res.     Publication Date:  2013 Nov 
Date Detail:
Created Date:  2013-12-09     Completed Date:  2014-03-27     Revised Date:  2014-07-22    
Medline Journal Info:
Nlm Unique ID:  9312706     Medline TA:  Arch Med Res     Country:  United States    
Other Details:
Languages:  eng     Pagination:  616-22     Citation Subset:  IM    
Copyright Information:
Copyright © 2013 IMSS. Published by Elsevier Inc. All rights reserved.
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MeSH Terms
Gene Expression Regulation
Heart Ventricles / chemistry,  physiopathology
MicroRNAs / biosynthesis,  physiology*
Myocardium / chemistry*,  metabolism*
Rats, Wistar
Thyroid Hormones / metabolism,  physiology*
Transforming Growth Factor beta / biosynthesis,  genetics,  physiology
Ventricular Remodeling / physiology*
Reg. No./Substance:
0/MIRN208 microRNA, rat; 0/MicroRNAs; 0/Thyroid Hormones; 0/Transforming Growth Factor beta
Comment In:
Arch Med Res. 2014 May;45(4):356   [PMID:  24751330 ]
Arch Med Res. 2014 May;45(4):357   [PMID:  24751332 ]

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