Document Detail


Role of Survivin in cytokinesis revealed by a separation-of-function allele.
MedLine Citation:
PMID:  21865602     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The chromosomal passenger complex (CPC), containing Aurora B kinase, Inner Centromere Protein, Survivin, and Borealin, regulates chromosome condensation and interaction between kinetochores and microtubules at metaphase, then relocalizes to midzone microtubules at anaphase and regulates central spindle organization and cytokinesis. However, the precise role(s) played by the CPC in anaphase have been obscured by its prior functions in metaphase. Here we identify a missense allele of Drosophila Survivin that allows CPC localization and function during metaphase but not cytokinesis. Analysis of mutant cells showed that Survivin is essential to target the CPC and the mitotic kinesin-like protein 1 orthologue Pavarotti (Pav) to the central spindle and equatorial cell cortex during anaphase in both larval neuroblasts and spermatocytes. Survivin also enabled localization of Polo kinase and Rho at the equatorial cortex in spermatocytes, critical for contractile ring assembly. In neuroblasts, in contrast, Survivin function was not required for localization of Rho, Polo, or Myosin II to a broad equatorial cortical band but was required for Myosin II to transition to a compact, fully constricted ring. Analysis of this "separation-of-function" allele demonstrates the direct role of Survivin and the CPC in cytokinesis and highlights striking differences in regulation of cytokinesis in different cell systems.
Authors:
Edith Szafer-Glusman; Margaret T Fuller; Maria Grazia Giansanti
Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't     Date:  2011-08-24
Journal Detail:
Title:  Molecular biology of the cell     Volume:  22     ISSN:  1939-4586     ISO Abbreviation:  Mol. Biol. Cell     Publication Date:  2011 Oct 
Date Detail:
Created Date:  2011-10-14     Completed Date:  2012-02-14     Revised Date:  2013-06-27    
Medline Journal Info:
Nlm Unique ID:  9201390     Medline TA:  Mol Biol Cell     Country:  United States    
Other Details:
Languages:  eng     Pagination:  3779-90     Citation Subset:  IM    
Affiliation:
Department of Developmental Biology and Genetics, Stanford University School of Medicine, Stanford, CA 94305, USA.
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MeSH Terms
Descriptor/Qualifier:
Alleles
Anaphase / physiology*
Animals
Chromosome Segregation / physiology
Chromosomes / chemistry,  metabolism,  ultrastructure
Cytokinesis / physiology
Drosophila Proteins / genetics,  metabolism*
Drosophila melanogaster / genetics,  metabolism*
Inhibitor of Apoptosis Proteins / genetics,  metabolism*
Kinetochores / metabolism,  ultrastructure
Larva / genetics,  metabolism*
Male
Microscopy, Fluorescence
Microtubule-Associated Proteins / genetics,  metabolism*
Mitotic Spindle Apparatus / physiology,  ultrastructure
Mutation
Species Specificity
Spermatocytes / cytology,  metabolism*
Video Recording
Grant Support
ID/Acronym/Agency:
54 HD068158/HD/NICHD NIH HHS
Chemical
Reg. No./Substance:
0/Drosophila Proteins; 0/Inhibitor of Apoptosis Proteins; 0/Microtubule-Associated Proteins; 0/Pavarotti protein, Drosophila; 0/Survivin protein, Drosophila; 0/kinesin-like protein 1
Comments/Corrections

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