Document Detail

A role for separase in the regulation of RAB-11-positive vesicles at the cleavage furrow and midbody.
MedLine Citation:
PMID:  20116245     Owner:  NLM     Status:  MEDLINE    
Cell division requires coordinated regulation of chromosome segregation and cytokinesis. Although much is known about the function of the protease separase in promoting sister chromosome separation, the role of separase during cytokinesis is unclear. We show that separase localizes to the ingressing furrow and midbody during cytokinesis in the C. elegans embryo. Loss of separase function during the early mitotic divisions causes cytokinesis failure that is not due to eggshell defects or chromosome nondisjunction. Moreover, depletion of separase causes the accumulation of RAB-11-positive vesicles at the cleavage furrow and midbody that is not a consequence of chromosome nondisjunction, but is mimicked by depletion of vesicle fusion machinery. Collectively, these data indicate that separase is required for cytokinesis by regulating the incorporation of RAB-11-positive vesicles into the plasma membrane at the cleavage furrow and midbody.
Joshua N Bembenek; John G White; Yixian Zheng
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't     Date:  2010-01-28
Journal Detail:
Title:  Current biology : CB     Volume:  20     ISSN:  1879-0445     ISO Abbreviation:  Curr. Biol.     Publication Date:  2010 Feb 
Date Detail:
Created Date:  2010-02-10     Completed Date:  2010-05-06     Revised Date:  2013-05-31    
Medline Journal Info:
Nlm Unique ID:  9107782     Medline TA:  Curr Biol     Country:  England    
Other Details:
Languages:  eng     Pagination:  259-64     Citation Subset:  IM    
University of Wisconsin-Madison, Laboratory of Molecular Biology, 1525 Linden Drive, Madison, WI 53706, USA. <>
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MeSH Terms
Animals, Genetically Modified
Caenorhabditis elegans / cytology,  embryology,  genetics,  metabolism
Caenorhabditis elegans Proteins / genetics,  metabolism*
Chromosome Segregation / physiology
Cytokinesis / physiology*
Recombinant Fusion Proteins / genetics,  metabolism
rab GTP-Binding Proteins / metabolism*
Grant Support
5F32 GM076867-02/GM/NIGMS NIH HHS; F32 GM076867-02S1/GM/NIGMS NIH HHS; R01 GM56312/GM/NIGMS NIH HHS; R01 GM7583/GM/NIGMS NIH HHS; //Howard Hughes Medical Institute
Reg. No./Substance:
0/Caenorhabditis elegans Proteins; 0/Recombinant Fusion Proteins; EC 3.4.-/SEP-1 protein, C elegans; EC 3.6.1.-/rab GTP-Binding Proteins; EC 3.6.1.-/rab11 protein

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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